TMEFF2 sirna

TMEFF2 siRNA (Human)

Cat. Num. AAA8237441

• Gene Names: TMEFF2; TR; HPP1; TPEF; TR-2; TENB2; CT120.2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TMEFF2; TMEFF2 siRNA (Human); HPP1; TENB2; TPEF; Tomoregulin-2; TR-2; Hyperplastic polyposis protein 1; Transmembrane protein with EGF-like and two follistatin-like domains; TMEFF2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TMEFF2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 175

• Applicable Applications for TMEFF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TMEFF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TMEFF2 sirna

siRNA to inhibit TMEFF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 762205498
• NCBI GeneID: 23671
• NCBI Accession #: NP_001292063.1
• NCBI GenBank Nucleotide #: NM_001305134.1
• UniProt Accession #: Q9UIK5; Q2FA44; Q4ZFW4; Q53H90; Q53RE1; Q8N2R5; Q9NR15; Q9NSS5; Q9P2Y9; Q9UK65
• Molecular Weight: 19,472 Da
• NCBI Official Full Name: tomoregulin-2 isoform 2
• NCBI Official Synonym Full Names: transmembrane protein with EGF-like and two follistatin-like domains 2
• NCBI Official Symbol: TMEFF2
• NCBI Official Synonym Symbols: TR; HPP1; TPEF; TR-2; TENB2; CT120.2
• NCBI Protein Information: tomoregulin-2
• UniProt Protein Name: Tomoregulin-2
• Protein Family: Tomoregulin
• UniProt Gene Name: TMEFF2
• UniProt Synonym Gene Names: HPP1; TENB2; TPEF; TR-2
• UniProt Entry Name: TEFF2_HUMAN

NCBI Description

This gene encodes a member of the tomoregulin family of transmembrane proteins. This protein has been shown to function as both an oncogene and a tumor suppressor depending on the cellular context and may regulate prostate cancer cell invasion. Multiple soluble forms of this protein have been identified that arise from both an alternative splice variant and ectodomain shedding. Additionally, this gene has been found to be hypermethylated in multiple cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]

Uniprot Description

TMEFF2: may be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation. A member of the Cancer-Testis Antigen (CTA) superfamily. CTAs may play roles in embryonal development and tumor transformation or aspects of tumor progression. CTAs were once thought to be silenced in most normal adult tissues, with limited expression in fetal, placental, testis, and ovarian cells. These proteins are now known to be aberrantly expressed in various cancers and many are capable of eliciting humoral and cellular immune responses. Highly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques. Down-regulated in tumor cell lines in response to a high level of methylation in the 5' region. The CpG island methylation correlates with TMEFF2 silencing in tumor cell lines. Belongs to the tomoregulin family. Three isoforms of the human protein are produced by alternative splicing. Isoform three is expressed weakly in testis and at high levels in normal and cancerous prostate. Note: This description may include information from RefSeq and UniProtKB

Protein type: Cancer Testis Antigen (CTA); Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q32.3

Cellular Component: extracellular region; integral to membrane