ALDH9A1 sirna

ALDH9A1 siRNA (Human)

Cat. Num. AAA8237109

• Gene Names: ALDH9A1; E3; ALDH4; ALDH7; ALDH9; TMABADH
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALDH9A1; ALDH9A1 siRNA (Human); ALDH4; ALDH7; ALDH9; 4-trimethylaminobutyraldehyde dehydrogenase; TMABADH; Aldehyde dehydrogenase E3 isozyme; Aldehyde dehydrogenase family 9 member A1; Gamma-aminobutyraldehyde dehydrogenase; R-aminobutyraldehyde dehydrogenase; ALDH9A1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ALDH9A1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 424

• Applicable Applications for ALDH9A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ALDH9A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALDH9A1 sirna

siRNA to inhibit ALDH9A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 115387104
• NCBI GeneID: 223
• NCBI Accession #: NP_000687.3
• NCBI GenBank Nucleotide #: NM_000696.3
• UniProt Accession #: P49189; Q5VV90; Q6LCL1; Q9NZT7; B2R6X1; B4DXY7
• Molecular Weight: 46,455 Da
• NCBI Official Full Name: 4-trimethylaminobutyraldehyde dehydrogenase
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 9 family, member A1
• NCBI Official Symbol: ALDH9A1
• NCBI Official Synonym Symbols: E3; ALDH4; ALDH7; ALDH9; TMABADH
• NCBI Protein Information: 4-trimethylaminobutyraldehyde dehydrogenase
• UniProt Protein Name: 4-trimethylaminobutyraldehyde dehydrogenase
• Protein Family: Aldehyde dehydrogenase family
• UniProt Gene Name: ALDH9A1
• UniProt Synonym Gene Names: ALDH4; ALDH7; ALDH9; TMABADH
• UniProt Entry Name: AL9A1_HUMAN

NCBI Description

This protein belongs to the aldehyde dehydrogenase family of proteins. It has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. The enzyme catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid (GABA). This isozyme is a tetramer of identical 54-kD subunits. [provided by RefSeq, Jul 2008]

Uniprot Description

ALDH9A1: Converts gamma-trimethylaminobutyraldehyde into gamma- butyrobetaine. Catalyzes the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction. Belongs to the aldehyde dehydrogenase family.

Protein type: Amino Acid Metabolism - histidine; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - lysine degradation; Amino Acid Metabolism - tryptophan; EC 1.2.1.3; Lipid Metabolism - fatty acid; Amino Acid Metabolism - arginine and proline; Carbohydrate Metabolism - pyruvate; EC 1.2.1.47; Oxidoreductase; Other Amino Acids Metabolism - beta-alanine; Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - butanoate; Mitochondrial; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: mitochondrion; cytoplasm; cytosol

Molecular Function: 1-pyrroline dehydrogenase activity; amine binding; aldehyde dehydrogenase (NAD) activity; protein homodimerization activity; aminobutyraldehyde dehydrogenase activity; 3-chloroallyl aldehyde dehydrogenase activity; 4-trimethylammoniobutyraldehyde dehydrogenase activity; NAD binding

Biological Process: hormone metabolic process; aldehyde metabolic process; liver development; kidney development; carnitine biosynthetic process; neurotransmitter biosynthetic process