GDF6 sirna

GDF6 siRNA (Mouse)

Cat. Num. AAA8236943

• Gene Names: Gdf6; BMP13; GDF16; BMP-13
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GDF6; GDF6 siRNA (Mouse); BMP13; GDF-6; GDF16; Growth/differentiation factor 6; Bone morphogenetic protein 13; BMP-13; Growth/differentiation factor 16; GDF6 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GDF6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 454

• Applicable Applications for GDF6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GDF6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Ships on blue ice. Store at -20 degree C for one year.

Related Product Information for GDF6 sirna

siRNA to inhibit GDF6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 41529818
• NCBI GeneID: 242316
• NCBI Accession #: NP_038554.1
• NCBI GenBank Nucleotide #: NM_013526.1
• UniProt Accession #: P43028; Q70UT4
• Molecular Weight: 50,942 Da
• NCBI Official Full Name: growth/differentiation factor 6
• NCBI Official Synonym Full Names: growth differentiation factor 6
• NCBI Official Symbol: Gdf6
• NCBI Official Synonym Symbols: BMP13; GDF16; BMP-13
• NCBI Protein Information: growth/differentiation factor 6
• UniProt Protein Name: Growth/differentiation factor 6
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: Gdf6
• UniProt Synonym Gene Names: Bmp13; Gdf-6; Gdf16; GDF-6; BMP-13
• UniProt Entry Name: GDF6_MOUSE

Uniprot Description

GDF6: Required for normal formation of bones and joints in the limbs, skull, and axial skeleton. Plays a key role in establishing boundaries between skeletal elements during development. Defects in GDF6 are the cause of Klippel-Feil syndrome type 1 (KFS1). A skeletal disorder characterized by congenital fusion of cervical vertebrae. It is due to a failure in the normal segmentation of vertebrae during the early weeks of fetal development. The clinical triad consists of short neck, low posterior hairline, and limited neck movement. Deafness is a well- known feature of KFS and may be of sensorineural, conductive, or mixed type. A chromosomal aberration involving GDF6 has been found in a patient with Klippel-Feil syndrome (KFS). Paracentric inv(8)(q22;2q23.3). Defects in GDF6 are the cause of microphthalmia isolated type 4 (MCOP4). A disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Belongs to the TGF-beta family.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide; Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: protein homodimerization activity; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; BMP signaling pathway; apoptosis; regulation of MAPKKK cascade; multicellular organismal development; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; positive regulation of neuron differentiation; growth

Disease: Coloboma, Ocular, Autosomal Dominant