SUPT4H1A sirna

SUPT4H1A siRNA (Mouse)

Cat. Num. AAA8236885

• Gene Names: Supt4a; Supt4h; Supt4h1; AL022777; Supt4h1a
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SUPT4H1A; SUPT4H1A siRNA (Mouse); SPT4H; SUPT4A; SUPT4H; Transcription elongation factor SPT4-A; DRB sensitivity-inducing factor small subunit 1; DSIF small subunit 1; Transcription elongation factor SPT4 1; SUPT4H1A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SUPT4H1A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 117

• Applicable Applications for SUPT4H1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SUPT4H1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SUPT4H1A sirna

siRNA to inhibit SUPT4H1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6678181
• NCBI GeneID: 20922
• NCBI Accession #: NP_033322.1
• NCBI GenBank Nucleotide #: NM_009296.1
• UniProt Accession #: P63271; Q16550; Q3TIA2; Q5NCP8; Q5NCP9; Q5NCQ0; Q62387; B2KGH6
• Molecular Weight: 13,193 Da
• NCBI Official Full Name: transcription elongation factor SPT4-A
• NCBI Official Synonym Full Names: suppressor of Ty 4A
• NCBI Official Symbol: Supt4a
• NCBI Official Synonym Symbols: Supt4h; Supt4h1; AL022777; Supt4h1a
• NCBI Protein Information: transcription elongation factor SPT4-A
• UniProt Protein Name: Transcription elongation factor SPT4-A
• Protein Family: Transcription elongation factor
• UniProt Gene Name: Supt4h1a
• UniProt Synonym Gene Names: Spt4h; Supt4a; Supt4h; DSIF small subunit 1
• UniProt Entry Name: SPT4A_MOUSE

Uniprot Description

SUPT4H1: Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV- 1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. Belongs to the SPT4 family.

Cellular Component: nucleoplasm; nucleus

Molecular Function: zinc ion binding; protein heterodimerization activity; metal ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of RNA elongation; RNA elongation from RNA polymerase II promoter; negative regulation of RNA elongation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter