SH2D2A sirna

SH2D2A siRNA (Human)

Cat. Num. AAA8236409

• Gene Names: SH2D2A; SCAP; TSAD; VRAP; F2771
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SH2D2A; SH2D2A siRNA (Human); SCAP; TSAD; VRAP; SH2 domain-containing protein 2A; SH2 domain-containing adapter protein; T cell-specific adapter protein; TSAd; VEGF receptor-associated protein; SH2D2A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SH2D2A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 371

• Applicable Applications for SH2D2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SH2D2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SH2D2A sirna

siRNA to inhibit SH2D2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 239787758
• NCBI GeneID: 9047
• NCBI Accession #: NP_001154914.1
• NCBI GenBank Nucleotide #: NM_001161442.1
• UniProt Accession #: Q9NP31; O43817; Q5UBZ1; Q5VZS4; Q5VZS5; Q9UPA7
• Molecular Weight: 40,954 Da
• NCBI Official Full Name: SH2 domain-containing protein 2A isoform 3
• NCBI Official Synonym Full Names: SH2 domain containing 2A
• NCBI Official Symbol: SH2D2A
• NCBI Official Synonym Symbols: SCAP; TSAD; VRAP; F2771
• NCBI Protein Information: SH2 domain-containing protein 2A
• UniProt Protein Name: SH2 domain-containing protein 2A
• Protein Family: SH2 domain-containing protein
• UniProt Gene Name: SH2D2A
• UniProt Synonym Gene Names: SCAP; TSAD; VRAP; TSAd
• UniProt Entry Name: SH22A_HUMAN

NCBI Description

This gene encodes an adaptor protein thought to function in T-cell signal transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

TSAd: an adaptor protein that may facilitate and regulate interaction of KDR with effector proteins important to endothelial cell survival and proliferation. May play a role in the CD4-p56- LCK-dependent signal transduction pathway in T cells. Could also play an important role in normal and pathological angiogenesis.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; SH3 domain binding

Biological Process: cell proliferation; positive regulation of signal transduction; angiogenesis; cell differentiation; signal transduction; vascular endothelial growth factor receptor signaling pathway