RRAGA sirna

RRAGA siRNA (Human)

Cat. Num. AAA8235935

• Gene Names: RRAGA; FIP1; RAGA; FIP-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RRAGA; RRAGA siRNA (Human); Ras-related GTP-binding protein A; Rag A; RagA; Adenovirus E3 14.7 kDa-interacting protein 1; FIP-1; RRAGA sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RRAGA siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 313

• Applicable Applications for RRAGA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RRAGA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RRAGA sirna

siRNA to inhibit RRAGA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5729999
• NCBI GeneID: 10670
• NCBI Accession #: NP_006561.1
• NCBI GenBank Nucleotide #: NM_006570.4
• UniProt Accession #: Q7L523; O00290; Q15347; B2R7L1
• Molecular Weight: 36,566 Da
• NCBI Official Full Name: ras-related GTP-binding protein A
• NCBI Official Synonym Full Names: Ras-related GTP binding A
• NCBI Official Symbol: RRAGA
• NCBI Official Synonym Symbols: FIP1; RAGA; FIP-1
• NCBI Protein Information: ras-related GTP-binding protein A
• UniProt Protein Name: Ras-related GTP-binding protein A
• UniProt Gene Name: RRAGA
• UniProt Synonym Gene Names: Rag A; RagA
• UniProt Entry Name: RRAGA_HUMAN

Uniprot Description

RRAGA: Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF- alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. Can occur as a homodimer, or form a heterodimer with RRAGC or RRAGD in a sequence-independent manner. Binds GTP. The GTP-bound form of RRAGA interacts with NOL8. Interacts with adenovirus E3 14.7 kDa protein. Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain. Belongs to the GTR/RAG GTP-binding protein family.

Protein type: G protein regulator, misc.

Chromosomal Location of Human Ortholog: 9p22.1

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; lysosome; lysosomal membrane; cytoplasm; nucleus

Molecular Function: GTPase activity; protein binding; protein homodimerization activity; GDP binding; GTP binding; protein heterodimerization activity; phosphoprotein binding

Biological Process: cell death; apoptosis; metabolic process; virus-host interaction; positive regulation of cytolysis; regulation of autophagy; cellular response to starvation; positive regulation of TOR signaling pathway