MYRF sirna

MYRF siRNA (Human)

Cat. Num. AAA8235917

• Gene Names: MYRF; MRF; Ndt80; pqn-47; C11orf9
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYRF; MYRF siRNA (Human); C11orf9; KIAA0954; MRF; Myelin regulatory factor; Myelin gene regulatory factor; MYRF sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MYRF siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,111

• Applicable Applications for MYRF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MYRF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYRF sirna

siRNA to inhibit MYRF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 188528652
• NCBI GeneID: 745
• NCBI Accession #: NP_001120864.1
• NCBI GenBank Nucleotide #: NM_001127392.2
• UniProt Accession #: Q9Y2G1; O43582; Q9P1Q6
• Molecular Weight: 120,225 Da
• NCBI Official Full Name: myelin regulatory factor isoform 2
• NCBI Official Synonym Full Names: myelin regulatory factor
• NCBI Official Symbol: MYRF
• NCBI Official Synonym Symbols: MRF; Ndt80; pqn-47; C11orf9
• NCBI Protein Information: myelin regulatory factor
• UniProt Protein Name: Myelin regulatory factor
• Protein Family: Myelin regulatory factor
• UniProt Gene Name: MYRF
• UniProt Synonym Gene Names: C11orf9; KIAA0954; MRF
• UniProt Entry Name: MRF_HUMAN

NCBI Description

This gene encodes a transcription factor that is required for central nervous system myelination and may regulate oligodendrocyte differentiation. It is thought to act by increasing the expression of genes that effect myelin production but may also directly promote myelin gene expression. Loss of a similar gene in mouse models results in severe demyelination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

MRF: Transcription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes. Belongs to the MRF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12-q13.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane; nucleus

Molecular Function: peptidase activity; DNA binding; transcription factor activity

Biological Process: positive regulation of myelination; transcription, DNA-dependent; myelin maintenance in the central nervous system; myelination in the central nervous system; positive regulation of transcription, DNA-dependent; oligodendrocyte differentiation; oligodendrocyte development; proteolysis