CREB5 sirna

CREB5 siRNA (Human)

Cat. Num. AAA8235819

• Gene Names: CREB5; CREB-5; CRE-BPA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CREB5; CREB5 siRNA (Human); CREBPA; Cyclic AMP-responsive element-binding protein 5; CREB-5; cAMP-responsive element-binding protein 5; CRE-BPa; CREB5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CREB5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 369

• Applicable Applications for CREB5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CREB5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CREB5 sirna

siRNA to inhibit CREB5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 59938776
• NCBI GeneID: 9586
• NCBI Accession #: NP_001011666.1
• NCBI GenBank Nucleotide #: NM_001011666.2
• UniProt Accession #: Q02930; Q05886; Q06246; Q75N02; Q86UJ9; A8KA51; B4DU13; B5BUH3; C9JK47
• Molecular Weight: 41,161 Da
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 5 isoform delta
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 5
• NCBI Official Symbol: CREB5
• NCBI Official Synonym Symbols: CREB-5; CRE-BPA
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 5
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 5
• Protein Family: Cyclic AMP-responsive element-binding protein
• UniProt Gene Name: CREB5
• UniProt Synonym Gene Names: CREBPA; CREB-5; cAMP-responsive element-binding protein 5
• UniProt Entry Name: CREB5_HUMAN

NCBI Description

The product of this gene belongs to the CRE (cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The encoded protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

CREB5: Binds to the cAMP response element and activates transcription. Belongs to the bZIP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 7p15.1

Cellular Component: nucleus

Molecular Function: protein binding; sequence-specific DNA binding; metal ion binding; transcription factor activity

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter