MED30 sirna

MED30 siRNA (Human)

Cat. Num. AAA8235021

• Gene Names: MED30; MED30S; THRAP6; TRAP25
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MED30; MED30 siRNA (Human); THRAP6; TRAP25; Mediator of RNA polymerase II transcription subunit 30; Mediator complex subunit 30; TRAP/Mediator complex component TRAP25; Thyroid hormone receptor-associated protein 6; Thyroid hormone receptor-associated protein complex 25 kDa component; Trap25; MED30 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MED30 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 143

• Applicable Applications for MED30 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MED30 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MED30 sirna

siRNA to inhibit MED30 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 545479145
• NCBI GeneID: 90390
• NCBI Accession #: NP_001269915.1
• NCBI GenBank Nucleotide #: NM_001282986.1
• UniProt Accession #: Q96HR3; C6GKU9
• Molecular Weight: 16,279 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 30 isoform 2
• NCBI Official Synonym Full Names: mediator complex subunit 30
• NCBI Official Symbol: MED30
• NCBI Official Synonym Symbols: MED30S; THRAP6; TRAP25
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 30
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 30
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED30
• UniProt Synonym Gene Names: THRAP6; TRAP25; Trap25
• UniProt Entry Name: MED30_HUMAN

NCBI Description

The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM, Nov 2010]

Uniprot Description

MED30: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 30 family.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 8q24.11

Cellular Component: nucleoplasm; Srb-mediator complex; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription cofactor activity; receptor activity; thyroid hormone receptor binding

Biological Process: regulation of transcription from RNA polymerase II promoter; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; stem cell maintenance; gene expression