PEBP1 sirna

PEBP1 siRNA (Human)

Cat. Num. AAA8235011

• Gene Names: PEBP1; PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PEBP1; PEBP1 siRNA (Human); PBP; PEBP; Phosphatidylethanolamine-binding protein 1; PEBP-1; HCNPpp; Neuropolypeptide h3; Prostatic-binding protein; Raf kinase inhibitor protein; RKIP; PEBP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PEBP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 187

• Applicable Applications for PEBP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PEBP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PEBP1 sirna

siRNA to inhibit PEBP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4505621
• NCBI GeneID: 5037
• NCBI Accession #: NP_002558.1
• NCBI GenBank Nucleotide #: NM_002567.3
• UniProt Accession #: P30086; B2R4S1
• Molecular Weight: 21,057 Da
• NCBI Official Full Name: phosphatidylethanolamine-binding protein 1
• NCBI Official Synonym Full Names: phosphatidylethanolamine binding protein 1
• NCBI Official Symbol: PEBP1
• NCBI Official Synonym Symbols: PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34
• NCBI Protein Information: phosphatidylethanolamine-binding protein 1
• UniProt Protein Name: Phosphatidylethanolamine-binding protein 1
• Protein Family: Phosphatidylethanolamine-binding protein
• UniProt Gene Name: PEBP1
• UniProt Synonym Gene Names: PBP; PEBP; PEBP-1; RKIP; HCNP
• UniProt Entry Name: PEBP1_HUMAN

NCBI Description

This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Jul 2015]

Uniprot Description

RKIP: a metastasis suppressor that regulates extracellular matrix remodeling and tumor survival. Binds to and inhibits Raf-1. Phosphorylation by classic and atypical but not novel PKC isoforms dissociates this complex, relieving inhibition of the Raf/MAP kinase signaling cascade. Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. Cleaved into hippocampal cholinergic neurostimulating peptide (HCMP) that appears to be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Expressed in brain. Increased expression in aged senescence-accelerated mice.

Protein type: Protein kinase, regulatory subunit; Lipid-binding

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: cytoplasm; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; phosphatidylethanolamine binding; protein kinase binding; ATP binding