PDP1 blocking peptide

PDP1 Blocking Peptide

Cat. Num. AAA8234888

• Gene Names: PDP1; PDH; PDP; PDPC; PPM2C
• Reactivity: Human, Mouse, Bovine
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: PDP1; PDP1 Blocking Peptide; PDP; PPM2C; [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1; mitochondrial; PDP 1; Protein phosphatase 2C; Pyruvate dehydrogenase phosphatase catalytic subunit 1; PDPC 1; PDP1 blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Bovine
• Purity/Purification: >85%
• Form/Format: Lyophilized powder
• Sequence Length: 562

• Applicable Applications for PDP1 blocking peptide: Blocking (BL)
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Directions for Use: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDP1 blocking peptide

The peptide is used to block Anti-PDP1 Antibody reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 239985422
• NCBI GeneID: 54704
• NCBI Accession #: NP_001155251.1
• NCBI GenBank Nucleotide #: NM_001161779.1
• UniProt Accession #: Q9P0J1; Q5U5K1; B3KX71; J3KPU0
• Molecular Weight: 63,695 Da
• NCBI Official Full Name: pyruvate dehyrogenase phosphatase catalytic subunit 1 isoform 2
• NCBI Official Synonym Full Names: pyruvate dehyrogenase phosphatase catalytic subunit 1
• NCBI Official Symbol: PDP1
• NCBI Official Synonym Symbols: PDH; PDP; PDPC; PPM2C
• NCBI Protein Information: pyruvate dehyrogenase phosphatase catalytic subunit 1
• UniProt Protein Name: [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
• Protein Family: PWWP domain-containing protein
• UniProt Gene Name: PDP1
• UniProt Synonym Gene Names: PDP; PPM2C; PDP 1; PDPC 1
• UniProt Entry Name: PDP1_HUMAN

NCBI Description

Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]

Uniprot Description

PDP1: a protein phosphoserine phosphatase associated with the mitochondrial matrix that activates phosphorylated pyruvate dehydrogenase complex by dephosphorylation. The PDPs play crucial roles in switching metabolic flux from glycolysis towards oxidative phosphorylation. Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex . PDP1 is composed of a catalytic subunit and a regulatory subunit (PDPR). Heterodimer of a catalytic subunit and a FAD protein of unknown function . Defects in PDP1 are the cause of pyruvate dehydrogenase phosphatase deficiency (PDP deficiency). PDP deficiency results in lactic acidosis leading to neurological dysfunction. Belongs to the PP2C family.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.43; Motility/polarity/chemotaxis; Mitochondrial

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: mitochondrial matrix

Molecular Function: [pyruvate dehydrogenase (lipoamide)] phosphatase activity; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: cellular metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; pyruvate metabolic process

Disease: Pyruvate Dehydrogenase Phosphatase Deficiency