IL13RA2 sirna

IL13RA2 siRNA (Mouse)

Cat. Num. AAA8234249

• Gene Names: Il13ra2; CD213a2; IL-13R-alpha-2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IL13RA2; IL13RA2 siRNA (Mouse); Interleukin-13 receptor subunit alpha-2; IL-13 receptor subunit alpha-2; IL-13R subunit alpha-2; IL-13R-alpha-2; IL-13RA2; CD antigen CD213a2; IL13RA2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: IL13RA2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 339

• Applicable Applications for IL13RA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse IL13RA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IL13RA2 sirna

siRNA to inhibit IL13RA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 807066377
• NCBI GeneID: 16165
• NCBI Accession #: NP_001292988.1
• NCBI GenBank Nucleotide #: NM_001306059.1
• UniProt Accession #: O88786; Q3V2V5; A3F812
• Molecular Weight: 39,429 Da
• NCBI Official Full Name: interleukin-13 receptor subunit alpha-2 isoform 2
• NCBI Official Synonym Full Names: interleukin 13 receptor, alpha 2
• NCBI Official Symbol: Il13ra2
• NCBI Official Synonym Symbols: CD213a2; IL-13R-alpha-2
• NCBI Protein Information: interleukin-13 receptor subunit alpha-2
• UniProt Protein Name: Interleukin-13 receptor subunit alpha-2
• Protein Family: Interleukin-13 receptor
• UniProt Gene Name: Il13ra2
• UniProt Synonym Gene Names: IL-13 receptor subunit alpha-2; IL-13R subunit alpha-2; IL-13R-alpha-2; IL-13RA2
• UniProt Entry Name: I13R2_MOUSE

NCBI Description

This gene encodes a receptor protein that binds to interleukin 13 (IL-13) with very high affinity. The encoded protein acts as a decoy receptor, and does not elicit any signal upon the binding of IL-13. Mice lacking the encoded protein exhibit increased levels of serum immunoglobulins, immune-dependent production of interferon gamma and, increased bone marrow macrophage progenitor frequency. Macrophages lacking the encoded protein release less nitric oxide and IL-12 in response to lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Uniprot Description

IL13RA2: a type I membrane protein that binds with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4). Lacks a cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13. A member of the Cancer-Testis Antigen (CTA) superfamily. CTAs may play roles in embryonal development and tumor transformation or aspects of tumor progression. CTAs were once thought to be silenced in most normal adult tissues, with limited expression in fetal, placental, testis, and ovarian cells. These proteins are now known to be aberrantly expressed in various cancers and many are capable of eliciting humoral and cellular immune responses. The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation. Often overexpressed in brain tumors. A potential vaccine targets for immunotherapy of glioma. Note: This description may include information from RefSeq and UniProtKB

Protein type: Receptor, cytokine; Cancer Testis Antigen (CTA); Membrane protein, integral

Cellular Component: membrane; integral to membrane; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity

Biological Process: negative regulation of mast cell degranulation; negative regulation of immunoglobulin production; immunoglobulin mediated immune response