CTDSPL sirna

CTDSPL siRNA (Mouse)

Cat. Num. AAA8234245

• Gene Names: Ctdspl; SCP3; HYA22; AI426263; 2810418J22Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTDSPL; CTDSPL siRNA (Mouse); NIF1; NIFL; CTD small phosphatase-like protein; CTDSP-like; Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3; NIF-like protein; Nuclear LIM interactor-interacting factor 1; NLI-interacting factor 1; Small C-terminal domain phosphatase 3; SCP3; Small CTD phosphatase 3; CTDSPL sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CTDSPL siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 276

• Applicable Applications for CTDSPL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CTDSPL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTDSPL sirna

siRNA to inhibit CTDSPL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 171460950
• NCBI GeneID: 69274
• NCBI Accession #: NP_598471.3
• NCBI GenBank Nucleotide #: NM_133710.3
• UniProt Accession #: P58465; Q52KL5
• Molecular Weight: 31,156 Da
• NCBI Official Full Name: CTD small phosphatase-like protein
• NCBI Official Synonym Full Names: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like
• NCBI Official Symbol: Ctdspl
• NCBI Official Synonym Symbols: SCP3; HYA22; AI426263; 2810418J22Rik
• NCBI Protein Information: CTD small phosphatase-like protein
• UniProt Protein Name: CTD small phosphatase-like protein
• Protein Family: CTD small phosphatase-like protein
• UniProt Gene Name: Ctdspl
• UniProt Synonym Gene Names: Nif1; Nifl; CTDSP-like; NLI-interacting factor 1; SCP3; Small CTD phosphatase 3
• UniProt Entry Name: CTDSL_MOUSE

Uniprot Description

CTDSPL: Preferentially catalyzes the dephosphorylation of 'Ser- 5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.16; Phosphatase

Cellular Component: nucleus

Molecular Function: hydrolase activity; metal ion binding; phosphoric monoester hydrolase activity; phosphoprotein phosphatase activity

Biological Process: negative regulation of protein amino acid phosphorylation