ISG20 sirna

ISG20 siRNA (Human)

Cat. Num. AAA8234070

• Gene Names: ISG20; CD25; HEM45
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ISG20; ISG20 siRNA (Human); HEM45; Interferon-stimulated gene 20 kDa protein; Estrogen-regulated transcript 45 protein; Promyelocytic leukemia nuclear body-associated protein ISG20; ISG20 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ISG20 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 181

• Applicable Applications for ISG20 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ISG20 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ISG20 sirna

siRNA to inhibit ISG20 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 735367255
• NCBI GeneID: 3669
• NCBI Accession #: NP_001290162.1
• NCBI GenBank Nucleotide #: NM_001303233.1
• UniProt Accession #: Q96AZ6; O00441; O00586
• Molecular Weight: 9,199 Da
• NCBI Official Full Name: interferon-stimulated gene 20 kDa protein isoform a
• NCBI Official Synonym Full Names: interferon stimulated exonuclease gene 20kDa
• NCBI Official Symbol: ISG20
• NCBI Official Synonym Symbols: CD25; HEM45
• NCBI Protein Information: interferon-stimulated gene 20 kDa protein
• UniProt Protein Name: Interferon-stimulated gene 20 kDa protein
• Protein Family: Interferon-stimulated gene 20 kDa protein
• UniProt Gene Name: ISG20
• UniProt Synonym Gene Names: HEM45
• UniProt Entry Name: ISG20_HUMAN

Uniprot Description

ISG20: Exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. Degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. Involved in the antiviral function of IFN against RNA viruses. Belongs to the exonuclease superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Deoxyribonuclease; EC 3.1.13.1; Ribonuclease

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: nucleoplasm; Cajal body; PML body; cytoplasm; nucleolus; nucleus

Molecular Function: exoribonuclease II activity; 3'-5'-exoribonuclease activity; U1 snRNA binding; metal ion binding; exonuclease activity; single-stranded DNA specific 3'-5' exodeoxyribonuclease activity; U2 snRNA binding

Biological Process: cell proliferation; negative regulation of viral genome replication; cytokine and chemokine mediated signaling pathway; response to virus; RNA catabolic process; DNA catabolic process, exonucleolytic; defense response to virus; rRNA processing