HLA-DOB blocking peptide

HLA-DOB Blocking Peptide

Cat. Num. AAA823404

• Gene Names: HLA-DOB; DOB
• Reactivity: Human, Mouse, Rat
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: HLA-DOB; HLA-DOB Blocking Peptide; HLA class II histocompatibility antigen; DO beta chain; MHC class II antigen DOB; HLA-DOB blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Rat
• Purity/Purification: >85%
• Form/Format: Lyophilized powder
• Sequence Length: 273

• Applicable Applications for HLA-DOB blocking peptide: Blocking (BL)
• Application Notes: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HLA-DOB blocking peptide

The peptide is used to block Anti-HLA-DOB Antibody reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 4504403
• NCBI GeneID: 3112
• NCBI Accession #: NP_002111.1
• NCBI GenBank Nucleotide #: NM_002120.3
• UniProt Accession #: P13765; Q29746; Q29825; Q6FHC2; B0V0Y0
• Molecular Weight: 30,822 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen, DO beta chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DO beta
• NCBI Official Symbol: HLA-DOB
• NCBI Official Synonym Symbols: DOB
• NCBI Protein Information: HLA class II histocompatibility antigen, DO beta chain; MHC class II antigen DOB
• UniProt Protein Name: HLA class II histocompatibility antigen, DO beta chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DOB
• UniProt Entry Name: DOB_HUMAN

NCBI Description

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DOB: Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: lysosomal membrane; lysosome; integral to membrane; endosome membrane; MHC class II protein complex

Molecular Function: MHC class II receptor activity

Biological Process: negative regulation of antigen processing and presentation of peptide antigen via MHC class II; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; signal transduction