SLC1A2 sirna

SLC1A2 siRNA (Human)

Cat. Num. AAA8234002

• Gene Names: SLC1A2; HBGT; EAAT2; GLT-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC1A2; SLC1A2 siRNA (Human); EAAT2; GLT1; Excitatory amino acid transporter 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2; SLC1A2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC1A2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 565

• Applicable Applications for SLC1A2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC1A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC1A2 sirna

siRNA to inhibit SLC1A2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 307746865
• NCBI GeneID: 6506
• NCBI Accession #: NP_001182657.1
• NCBI GenBank Nucleotide #: NM_001195728.2
• UniProt Accession #: P43004; Q14417; Q541G6; B4DQE9; U3KQQ4
• Molecular Weight: 61,008 Da
• NCBI Official Full Name: excitatory amino acid transporter 2 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 1 (glial high affinity glutamate transporter), member 2
• NCBI Official Symbol: SLC1A2
• NCBI Official Synonym Symbols: HBGT; EAAT2; GLT-1
• NCBI Protein Information: excitatory amino acid transporter 2
• UniProt Protein Name: Excitatory amino acid transporter 2
• Protein Family: Excitatory amino acid transporter
• UniProt Gene Name: SLC1A2
• UniProt Synonym Gene Names: EAAT2; GLT1
• UniProt Entry Name: EAA2_HUMAN

NCBI Description

This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Sep 2010]

Uniprot Description

GLT1: an integral membrane protein belonging to the sodium:dicarboxylate (SDF) symporter family. Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by co-transporting sodium.

Protein type: Transporter, SLC family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p13-p12

Cellular Component: membrane; integral to membrane; plasma membrane; axolemma

Molecular Function: protein binding; sodium:dicarboxylate symporter activity; glutamate:sodium symporter activity; L-glutamate transmembrane transporter activity

Biological Process: response to drug; multicellular organism growth; multicellular organismal aging; response to amino acid stimulus; visual behavior; positive regulation of glucose import; synaptic transmission; L-glutamate import; telencephalon development; adult behavior; cellular response to extracellular stimulus; response to wounding; ion transport; transmembrane transport