Rabbit anti-Human CDK7 Polyclonal Antibody | anti-CDK7 antibody

CDK7, Phosphorylated (Thr170) (Cell Division Protein Kinase 7, CDK-activating Kinase, CAK, TFIIH Basal Transcription Factor Complex Kinase Subunit, 39kD Protein Kinase, P39 Mo15, STK1, CAK1, MO15) (Biotin)

Cat. Num. AAA6468513

• Gene Names: CDK7; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: CDK7; Polyclonal Antibody; Phosphorylated (Thr170) (Cell Division Protein Kinase 7; CDK-activating Kinase; CAK; TFIIH Basal Transcription Factor Complex Kinase Subunit; 39kD Protein Kinase; P39 Mo15; STK1; CAK1; MO15) (Biotin); anti-CDK7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CDK7 phosphorylated at Thr170.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CDK7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr170 of human CDK7 (KLH).
• Conjugate: Biotin
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The CDK7-T170 Pab is used in Western blot to detect CDK7-T170 in Ramos tissue lysate.)

Dot Blot (DB)

(Dot blot analysis of Phospho-Cdk7-T170 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CDK7 antibody

Antibodies; Protein Kinases

References

1. Zhou, M., et al., Proc. Natl. Acad. Sci. USA 100(22):12666-12671 (2003). 2. Kino, T., et al., Biochem. Biophys. Res. Commun. 298(1):17-23 (2002). 3. Schneider, E., et al., Oncogene 21(33):5031-5037 (2002). 4. Nekhai, S., et al., Virology 266(2):246-256 (2000). 5. Zhou, M., et al., Mol. Cell. Biol. 20(14):5077-5086 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4502743
• NCBI GeneID: 1022
• NCBI Accession #: NP_001790
• NCBI GenBank Nucleotide #: NM_001799
• UniProt Accession #: P50613; Q9BS60; Q9UE19
• Molecular Weight: 39,038 Da
• NCBI Official Full Name: cyclin-dependent kinase 7
• NCBI Official Synonym Full Names: cyclin-dependent kinase 7
• NCBI Official Symbol: CDK7
• NCBI Official Synonym Symbols: CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
• NCBI Protein Information: cyclin-dependent kinase 7; 39 KDa protein kinase; CAK; CDK-activating kinase 1; TFIIH basal transcription factor complex kinase subunit; cell division protein kinase 7; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); homol
• UniProt Protein Name: Cyclin-dependent kinase 7
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK7
• UniProt Synonym Gene Names: CAK; CAK1; CDKN7; MO15; STK1; p39 Mo15
• UniProt Entry Name: CDK7_HUMAN

Uniprot Description

CDK7: a protein kinase of the CDK family. Forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). Activates the cyclin-associated kinases CDK1, -2, -4 and -6. An essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. Serves as a direct link between the regulation of transcription and the cell cycle. Phosphorylates and activates RNA polymerase II, allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; Protein kinase, CMGC; EC 2.7.11.23; CMGC group; CDK family; CDK7 subfamily; CDK/CDK7 subfamily

Chromosomal Location of Human Ortholog: 5q12.1

Cellular Component: nucleoplasm; mitochondrion; holo TFIIH complex; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: protein C-terminus binding; RNA polymerase subunit kinase activity; DNA-dependent ATPase activity; protein binding; androgen receptor binding; cyclin-dependent protein kinase activity; transcription coactivator activity; ATP binding; protein kinase activity

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of gene expression, epigenetic; mRNA capping; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; G2/M transition of mitotic cell cycle; cell cycle arrest; transcription initiation from RNA polymerase II promoter; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; DNA repair; termination of RNA polymerase I transcription; cell proliferation; nucleotide-excision repair; cell division; RNA elongation from RNA polymerase II promoter; androgen receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; transcription initiation from RNA polymerase I promoter; G1/S transition of mitotic cell cycle