RBBP5 sirna

RBBP5 siRNA (Human)

Cat. Num. AAA8233599

• Gene Names: RBBP5; RBQ3; SWD1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RBBP5; RBBP5 siRNA (Human); RBQ3; Retinoblastoma-binding protein 5; RBBP-5; Retinoblastoma-binding protein RBQ-3; RBBP5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RBBP5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 500

• Applicable Applications for RBBP5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RBBP5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RBBP5 sirna

siRNA to inhibit RBBP5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 300796323
• NCBI GeneID: 5929
• NCBI Accession #: NP_001180201.1
• NCBI GenBank Nucleotide #: NM_001193272.1
• UniProt Accession #: Q15291; Q7Z6D8; Q8NDZ7; A8K272
• Molecular Weight: 55,072 Da
• NCBI Official Full Name: retinoblastoma-binding protein 5 isoform 2
• NCBI Official Synonym Full Names: retinoblastoma binding protein 5
• NCBI Official Symbol: RBBP5
• NCBI Official Synonym Symbols: RBQ3; SWD1
• NCBI Protein Information: retinoblastoma-binding protein 5
• UniProt Protein Name: Retinoblastoma-binding protein 5
• Protein Family: Retinoblastoma-binding protein
• UniProt Gene Name: RBBP5
• UniProt Synonym Gene Names: RBQ3; RBBP-5
• UniProt Entry Name: RBBP5_HUMAN

NCBI Description

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

RBBP5: As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Core component of several methyltransferase-containing complexes including MLL1/MLL, ASCOM, MLL2/MLL3 and MLL3/MLL4. Each complex is at least composed of ASH2L, RBBP5, DPY30, WDR5, one or more specific histone methyltransferases (MLL, MLL2, MLL3 and MLL4), and the facultative components C16orf53/PA1, C17orf49, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with WDR5 and ASH2L; the interaction is direct. Interacts with WDR82 and SETD1A. Ubiquitously expressed. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; histone methyltransferase complex; nucleolus; nucleus

Molecular Function: protein binding; histone lysine N-methyltransferase activity (H3-K4 specific); methylated histone residue binding

Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to estrogen stimulus; histone H3-K4 methylation; response to DNA damage stimulus