VEGFD recombinant protein

VEGFD Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8118640

• Gene Names: FIGF; VEGFD; VEGF-D
• Purity: >95 % as determined by SDS-PAGE.
• Synonyms: VEGFD; VEGFD Protein; Human; Recombinant (His Tag); Human VEGF-D/VEGFD/FIGF Protein (His Tag); c-fos induced growth factor (vascular endothelial growth factor D); VEGF-D Protein; VEGFD recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 120aa
• Sequence: Phe93-Ser201

• Species: Human
• Tag: C-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Phe93
• Protein Construction: |A DNA sequence encoding the mature form of human VEGFD (NP_004460.1) corresponding to amino acid (Phe 93-Ser 201) was expressed with a C-terminal polyhistidine tag
• Activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 0.3-1.6ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 0.3-1.6 ug/mL.)

Related Product Information for VEGFD recombinant protein

Vascular endothelial growth factor D (VEGF-D), also known as C-fos induced growth factor (FIGF), belongs to the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. FIGF protein is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. FIGF protein is secreted as a non-covelent homodimer in an antiparallel fashion. Human FIGF protein is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin. FIGF protein is structurally and functionally similar to VEGF-C. Therefore, FIGF protein binds and activates VEGFR-2 (Flk1) and VEGFR-3 (Flt4) receptors, and may particularly be involved in cancers, such as breast cancer, epithelial ovarian carcinoma and so on.

References

Avantaggiato V, et al. (1998) Embryonic expression pattern of the murine figf gene, a growth factor belonging to platelet-derived growth factor/vascular endothelial growth factor family. Mech Dev. 73(2):221-4.Rocchigiani M, et al. (1998) Human FIGF: cloning, gene structure, and mapping to chromosome Xp22.1 between the PIGA and the GRPR genes. Genomics 47(2):207-16.Karpanen T, et al. (2008) VEGF-D: a modifier of embryonic lymphangiogenesis. Blood. 112(5): 1547-8.

NCBI and Uniprot Product Information

• NCBI GI #: 4758378
• NCBI GeneID: 2277
• NCBI Accession #: NP_004460.1
• NCBI GenBank Nucleotide #: NM_004469.4
• UniProt Accession #: O43915; B2R7Z3
• Molecular Weight: 40,444 Da
• NCBI Official Full Name: vascular endothelial growth factor D preproprotein
• NCBI Official Synonym Full Names: c-fos induced growth factor (vascular endothelial growth factor D)
• NCBI Official Symbol: FIGF
• NCBI Official Synonym Symbols: VEGFD; VEGF-D
• NCBI Protein Information: vascular endothelial growth factor D
• UniProt Protein Name: Vascular endothelial growth factor D
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: FIGF
• UniProt Synonym Gene Names: VEGFD; VEGF-D; FIGF
• UniProt Entry Name: VEGFD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C. Read-through transcription has been observed between this locus and the upstream PIR (GeneID 8544) locus. [provided by RefSeq, Feb 2011]

Uniprot Description

VEGFD: Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Belongs to the PDGF/VEGF growth factor family.

Protein type: Cytokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: Xp22.31

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: protein homodimerization activity; growth factor activity; platelet-derived growth factor receptor binding; vascular endothelial growth factor receptor 3 binding; vascular endothelial growth factor receptor binding; chemoattractant activity

Biological Process: cell proliferation; platelet activation; platelet degranulation; positive chemotaxis; positive regulation of cell division; positive regulation of cell proliferation; angiogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; cell differentiation; induction of positive chemotaxis