ACD sirna

ACD siRNA (Human)

Cat. Num. AAA8233512

• Gene Names: ACD; PIP1; PTOP; TPP1; TINT1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACD; ACD siRNA (Human); PIP1; PTOP; TINT1; TPP1; Adrenocortical dysplasia protein homolog; POT1 and TIN2-interacting protein; ACD sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ACD siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 544

• Applicable Applications for ACD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ACD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACD sirna

siRNA to inhibit ACD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 130978956
• NCBI GeneID: 65057
• NCBI Accession #: NP_001075955.1
• NCBI GenBank Nucleotide #: NM_001082486.1
• UniProt Accession #: Q96AP0; Q562H5; Q9H8F9
• Molecular Weight: 57,393 Da
• NCBI Official Full Name: adrenocortical dysplasia protein homolog isoform 1
• NCBI Official Synonym Full Names: adrenocortical dysplasia homolog (mouse)
• NCBI Official Symbol: ACD
• NCBI Official Synonym Symbols: PIP1; PTOP; TPP1; TINT1
• NCBI Protein Information: adrenocortical dysplasia protein homolog
• UniProt Protein Name: Adrenocortical dysplasia protein homolog
• Protein Family: ACD11 homolog protein
• UniProt Gene Name: ACD
• UniProt Synonym Gene Names: PIP1; PTOP; TINT1; TPP1
• UniProt Entry Name: ACD_HUMAN

NCBI Description

This gene encodes a protein that is involved in telomere function. This protein is one of six core proteins in the telosome/shelterin telomeric complex, which functions to maintain telomere length and to protect telomere ends. Through its interaction with other components, this protein plays a key role in the assembly and stabilization of this complex, and it mediates the access of telomerase to the telomere. Multiple transcript variants encoding different isoforms have been found for this gene. This gene, which is also referred to as TPP1, is distinct from the unrelated TPP1 gene on chromosome 11, which encodes tripeptidyl-peptidase I. [provided by RefSeq, Jul 2008]

Uniprot Description

ACD: Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Promotes binding of POT1 to single-stranded telomeric DNA. Modulates the inhibitory effects of POT1 on telomere elongation. The ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity. Plays a role in shelterin complex assembly. May play a role in organogenesis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: telomere assembly; intracellular protein transport; telomere capping; segmentation; positive regulation of telomerase activity; skeletal development; telomere maintenance; negative regulation of telomere maintenance via telomerase; urogenital system development; embryonic limb morphogenesis; protection from non-homologous end joining at telomere