RREB1 sirna

RREB1 siRNA (Mouse)

Cat. Num. AAA8233206

• Gene Names: Rreb1; AA414966; 1110037N09Rik; B930013M22Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RREB1; RREB1 siRNA (Mouse); Ras-responsive element-binding protein 1; RREB-1; RAS-responsive zinc finger transcription factor RREB; RREB1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RREB1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1291

• Applicable Applications for RREB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RREB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RREB1 sirna

siRNA to inhibit RREB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 85719305
• NCBI GeneID: 68750
• NCBI Accession #: NP_001034277.1
• NCBI GenBank Nucleotide #: NM_001039188.1
• UniProt Accession #: Q3UH06; Q3TB97; Q4ZE88; Q66JZ8; B8JJE2; B8JJE3
• Molecular Weight: 140,184 Da
• NCBI Official Full Name: ras-responsive element-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: ras responsive element binding protein 1
• NCBI Official Symbol: Rreb1
• NCBI Official Synonym Symbols: AA414966; 1110037N09Rik; B930013M22Rik
• NCBI Protein Information: ras-responsive element-binding protein 1
• UniProt Protein Name: Ras-responsive element-binding protein 1
• Protein Family: Ras-responsive element-binding protein
• UniProt Gene Name: Rreb1
• UniProt Synonym Gene Names: RREB-1
• UniProt Entry Name: RREB1_MOUSE

Uniprot Description

RREB1: Transcription factor that binds specifically to the RAS- responsive elements (RRE) of gene promoters. May be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. Represses the angiotensinogen gene. Negatively regulates the transcriptional activity of AR. Potentiates the transcriptional activity of NEUROD1. Belongs to the krueppel C2H2-type zinc-finger protein family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; C2H2-type zinc finger protein; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: DNA binding; nucleic acid binding; metal ion binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of mammary gland epithelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent