RALGDS sirna

RALGDS siRNA (Human)

Cat. Num. AAA8232818

• Gene Names: RALGDS; RGF; RGDS; RalGEF
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RALGDS; RALGDS siRNA (Human); KIAA1308; RGF; Ral guanine nucleotide dissociation stimulator; RalGDS; Ral guanine nucleotide exchange factor; RalGEF; RALGDS sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RALGDS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 859

• Applicable Applications for RALGDS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RALGDS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RALGDS sirna

siRNA to inhibit RALGDS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 108796058
• NCBI GeneID: 5900
• NCBI Accession #: NP_001035827.1
• NCBI GenBank Nucleotide #: NM_001042368.2
• UniProt Accession #: Q12967; Q5T7V4; Q6KH11; Q6PCE1; Q6ZSD5; Q9HAX7; Q9HAY1; Q9HCT1; Q9P2N8; B7Z753; E7ER93; E7ERZ0
• Molecular Weight: 99,325 Da
• NCBI Official Full Name: ral guanine nucleotide dissociation stimulator isoform 2
• NCBI Official Synonym Full Names: ral guanine nucleotide dissociation stimulator
• NCBI Official Symbol: RALGDS
• NCBI Official Synonym Symbols: RGF; RGDS; RalGEF
• NCBI Protein Information: ral guanine nucleotide dissociation stimulator
• UniProt Protein Name: Ral guanine nucleotide dissociation stimulator
• Protein Family: Ral guanine nucleotide dissociation stimulator
• UniProt Gene Name: RALGDS
• UniProt Synonym Gene Names: KIAA1308; RGF; RalGDS; RalGEF
• UniProt Entry Name: GNDS_HUMAN

NCBI Description

Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.[supplied by OMIM, Nov 2010]

Uniprot Description

RALGDS: Stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Ras

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytosol; nucleus; brush border

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity

Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; regulation of catalytic activity; Ras protein signal transduction; positive regulation of GTPase activity