SIRT1 sirna

SIRT1 siRNA (Mouse)

Cat. Num. AAA8232326

• Gene Names: Sirt1; Sir2; Sir2a; SIR2L1; AA673258; Sir2alpha
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SIRT1; SIRT1 siRNA (Mouse); SIR2L1; NAD-dependent protein deacetylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; SIR2alpha; Sir2; mSIR2a; SIRT1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SIRT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 737

• Applicable Applications for SIRT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SIRT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SIRT1 sirna

siRNA to inhibit SIRT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9790229
• NCBI GeneID: 93759
• NCBI Accession #: NP_062786.1
• NCBI GenBank Nucleotide #: NM_019812.2
• UniProt Accession #: Q923E4; Q9QXG8
• Molecular Weight: 59,875 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-1 isoform 1
• NCBI Official Synonym Full Names: sirtuin 1
• NCBI Official Symbol: Sirt1
• NCBI Official Synonym Symbols: Sir2; Sir2a; SIR2L1; AA673258; Sir2alpha
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-1
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-1
• Protein Family: NAD-dependent protein deacetylase sirtuin
• UniProt Gene Name: Sirt1
• UniProt Synonym Gene Names: Sir2l1; Sir2; mSIR2a; 75SirT1
• UniProt Entry Name: SIR1_MOUSE

Uniprot Description

SIRT1: an NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separate cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. Elevation of NAD(+)/NADP(+) ratio activates SIRT1. Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression. Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG. Involved in liver and muscle metabolism. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and ATG8. Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Widely expressed. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.-; Deacetylase; Nuclear receptor co-regulator; Apoptosis

Cellular Component: PML body; mitochondrion; cell; ESC/E(Z) complex; nuclear inner membrane; nuclear envelope; nuclear heterochromatin; cytosol; nucleoplasm; chromatin silencing complex; growth cone; axon; nuclear chromatin; cytoplasm; chromatin; nucleus

Molecular Function: identical protein binding; protein kinase B binding; histone binding; metal ion binding; UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity; transcription factor binding; N-acetylgalactosamine-6-phosphate deacetylase activity; N2-acetyl-L-lysine deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; protein N-terminal asparagine amidohydrolase activity; mitogen-activated protein kinase binding; HLH domain binding; (3,5-dichlorophenylurea)acetate amidohydrolase activity; protein C-terminus binding; deacetylase activity; protein domain specific binding; hydrolase activity; p53 binding; protein deacetylase activity; iprodione amidohydrolase activity; protein binding; indoleacetamide hydrolase activity; NAD-dependent histone deacetylase activity; bHLH transcription factor binding; histone deacetylase activity; transcription corepressor activity; O-succinylbenzoate synthase activity

Biological Process: muscle development; establishment and/or maintenance of chromatin architecture; positive regulation of apoptosis; multicellular organismal development; regulation of mitotic cell cycle; rhythmic process; protein ubiquitination; positive regulation of caspase activity; negative regulation of prostaglandin biosynthetic process; positive regulation of vasodilation; negative regulation of DNA damage response, signal transduction by p53 class mediator; behavioral response to starvation; positive regulation of adaptive immune response; positive regulation of histone H3-K9 methylation; negative regulation of growth hormone secretion; positive regulation of DNA repair; transcription, DNA-dependent; negative regulation of transcription factor activity; pyrimidine dimer repair via nucleotide-excision repair; positive regulation of heart rate; negative regulation of I-kappaB kinase/NF-kappaB cascade; ovulation from ovarian follicle; negative regulation of fat cell differentiation; cellular response to starvation; positive regulation of skeletal muscle cell proliferation; regulation of endodeoxyribonuclease activity; cholesterol homeostasis; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; inhibition of NF-kappaB transcription factor; maintenance of chromatin silencing; negative regulation of phosphorylation; regulation of protein import into nucleus, translocation; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; negative regulation of transcription, DNA-dependent; triacylglycerol mobilization; peptidyl-lysine acetylation; rRNA processing; negative regulation of apoptosis; chromatin silencing at rDNA; proteasomal ubiquitin-dependent protein catabolic process; establishment of chromatin silencing; apoptosis; negative regulation of transcription from RNA polymerase II promoter; response to insulin stimulus; DNA synthesis during DNA repair; positive regulation of gluconeogenesis; regulation of transcription, DNA-dependent; protein amino acid deacetylation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; circadian regulation of gene expression; angiogenesis; cell differentiation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; positive regulation of chromatin silencing; single strand break repair; negative regulation of TOR signaling pathway; negative regulation of protein kinase B signaling cascade; protein destabilization; histone deacetylation; negative regulation of tumor necrosis factor production; regulation of cell proliferation; negative regulation of helicase activity; response to hydrogen peroxide; fatty acid homeostasis; white fat cell differentiation; cell glucose homeostasis; spermatogenesis; positive regulation of protein amino acid phosphorylation; negative regulation of cell growth; positive regulation of insulin receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway; response to DNA damage stimulus; positive regulation of macroautophagy