THY1 sirna

THY1 siRNA (Rat)

Cat. Num. AAA8232074

• Gene Names: Thy1; CD7
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: THY1; THY1 siRNA (Rat); THY-1; Thy-1 membrane glycoprotein; Thy-1 antigen; CD antigen CD90; THY1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: THY1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 161

• Applicable Applications for THY1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat THY1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for THY1 sirna

siRNA to inhibit THY1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981654
• NCBI GeneID: 24832
• NCBI Accession #: NP_036805.1
• NCBI GenBank Nucleotide #: NM_012673.2
• UniProt Accession #: P01830
• Molecular Weight: 18,172 Da
• NCBI Official Full Name: thy-1 membrane glycoprotein preproprotein
• NCBI Official Synonym Full Names: Thy-1 cell surface antigen
• NCBI Official Symbol: Thy1
• NCBI Official Synonym Symbols: CD7
• NCBI Protein Information: thy-1 membrane glycoprotein
• UniProt Protein Name: Thy-1 membrane glycoprotein
• Protein Family: Thy-1 membrane glycoprotein
• UniProt Gene Name: Thy1
• UniProt Synonym Gene Names: Thy-1
• UniProt Entry Name: THY1_RAT

NCBI Description

cell surface glycoprotein antigen [RGD, Feb 2006]

Uniprot Description

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Cellular Component: anchored to external side of plasma membrane; growth cone; cell surface; focal adhesion; anchored to plasma membrane; endoplasmic reticulum; dendrite; apical plasma membrane; plasma membrane; cytosol; myelin sheath; lipid raft; external side of plasma membrane

Molecular Function: integrin binding; protein binding; enzyme binding; protein kinase binding

Biological Process: focal adhesion formation; negative regulation of T cell receptor signaling pathway; cytoskeleton organization and biogenesis; mast cell activation; T cell receptor signaling pathway; cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; retinal cone cell development; negative regulation of fibroblast proliferation; negative regulation of protein kinase activity; negative regulation of axonogenesis; positive regulation of release of sequestered calcium ion into cytosol; angiogenesis; positive regulation of T cell activation; negative regulation of cell migration; positive regulation of GTPase activity; negative regulation of apoptosis