CLIC4 sirna

CLIC4 siRNA (Mouse)

Cat. Num. AAA8231655

• Gene Names: Clic4; TU-74; mc3s5; D0Jmb3; mtCLIC
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CLIC4; CLIC4 siRNA (Mouse); Chloride intracellular channel protein 4; mc3s5/mtCLIC; CLIC4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CLIC4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 253

• Applicable Applications for CLIC4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CLIC4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CLIC4 sirna

siRNA to inhibit CLIC4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7304963
• NCBI GeneID: 29876
• NCBI Accession #: NP_038913.1
• NCBI GenBank Nucleotide #: NM_013885.2
• UniProt Accession #: Q9QYB1; Q8BMG5
• Molecular Weight: 28,729 Da
• NCBI Official Full Name: chloride intracellular channel protein 4
• NCBI Official Synonym Full Names: chloride intracellular channel 4 (mitochondrial)
• NCBI Official Symbol: Clic4
• NCBI Official Synonym Symbols: TU-74; mc3s5; D0Jmb3; mtCLIC
• NCBI Protein Information: chloride intracellular channel protein 4
• UniProt Protein Name: Chloride intracellular channel protein 4
• Protein Family: Chloride intracellular channel protein
• UniProt Gene Name: Clic4
• UniProt Synonym Gene Names: mc3s5/mtCLIC
• UniProt Entry Name: CLIC4_MOUSE

Uniprot Description

CLIC4: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell- surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical- basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Belongs to the chloride channel CLIC family.

Protein type: Membrane protein, integral

Cellular Component: centrosome; nuclear matrix; microvillus; cell surface; mitochondrion; cell; integral to membrane; intercellular junction; cytosol; microtubule cytoskeleton; membrane; apical part of cell; perinuclear region of cytoplasm; cytoplasm; plasma membrane; vacuole; midbody; cytoplasmic vesicle; intracellular; nucleus

Molecular Function: chloride channel activity; protein binding; protein complex binding; voltage-gated ion channel activity

Biological Process: keratinocyte differentiation; vacuolar acidification; fertilization; branching morphogenesis of a tube; apoptosis; transport; multicellular organism growth; ion transport; chloride transport; angiogenesis; endothelial cell morphogenesis; negative regulation of cell migration