EFEMP2 sirna

EFEMP2 siRNA (Mouse)

Cat. Num. AAA8231532

• Gene Names: Efemp2; MBP1; Fbln4; 0610011K11Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EFEMP2; EFEMP2 siRNA (Mouse); FBLN4; MBP1; EGF-containing fibulin-like extracellular matrix protein 2; Fibulin-4; FIBL-4; Mutant p53-binding protein 1; EFEMP2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EFEMP2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 443

• Applicable Applications for EFEMP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EFEMP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EFEMP2 sirna

siRNA to inhibit EFEMP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 256355190
• NCBI GeneID: 58859
• NCBI Accession #: NP_067449.3
• NCBI GenBank Nucleotide #: NM_021474.3
• UniProt Accession #: Q9WVJ9
• Molecular Weight: 49,425 Da
• NCBI Official Full Name: EGF-containing fibulin-like extracellular matrix protein 2 isoform 1
• NCBI Official Synonym Full Names: epidermal growth factor-containing fibulin-like extracellular matrix protein 2
• NCBI Official Symbol: Efemp2
• NCBI Official Synonym Symbols: MBP1; Fbln4; 0610011K11Rik
• NCBI Protein Information: EGF-containing fibulin-like extracellular matrix protein 2
• UniProt Protein Name: EGF-containing fibulin-like extracellular matrix protein 2
• Protein Family: EGF-containing fibulin-like extracellular matrix protein
• UniProt Gene Name: Efemp2
• UniProt Synonym Gene Names: Fbln4; Mbp1; FIBL-4
• UniProt Entry Name: FBLN4_MOUSE

Uniprot Description

EFEMP2: Defects in EFEMP2 are the cause of cutis laxa, autosomal recessive, type 1B (ARCL1B). A connective tissue disorder characterized by characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. The clinical spectrum of autosomal recessive cutis laxa is highly heterogeneous with respect to organ involvement and severity. ARCL1B features include emphysema, lethal pulmonary artery occlusion, aortic aneurysm, cardiopulmonary insufficiency, birth fractures, arachnodactyly, and fragility of blood vessels. Belongs to the fibulin family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: proteinaceous extracellular matrix; extracellular region; vesicle

Molecular Function: calcium ion binding