CDK10 sirna

CDK10 siRNA (Human)

Cat. Num. AAA8231529

• Gene Names: CDK10; PISSLRE
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDK10; CDK10 siRNA (Human); Cyclin-dependent kinase 10; Cell division protein kinase 10; Serine/threonine-protein kinase PISSLRE; CDK10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CDK10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 283

• Applicable Applications for CDK10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CDK10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDK10 sirna

siRNA to inhibit CDK10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 237858580
• NCBI GeneID: 8558
• NCBI Accession #: NP_001092003.2
• NCBI GenBank Nucleotide #: NM_001098533.2
• UniProt Accession #: Q15131; Q0VGZ7; Q15130; Q6PJC0; A8K370; A8K8I6; A8MXU6; B3KQJ3; B7Z420; D3DX82; D3DX83
• Molecular Weight: 32,662 Da
• NCBI Official Full Name: cyclin-dependent kinase 10 isoform c
• NCBI Official Synonym Full Names: cyclin-dependent kinase 10
• NCBI Official Symbol: CDK10
• NCBI Official Synonym Symbols: PISSLRE
• NCBI Protein Information: cyclin-dependent kinase 10
• UniProt Protein Name: Cyclin-dependent kinase 10
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK10
• UniProt Entry Name: CDK10_HUMAN

NCBI Description

The protein encoded by this gene belongs to the CDK subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. This kinase has been shown to play a role in cellular proliferation and its function is limited to cell cycle G2-M phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

CDK10: a CMGC kinase of the CDK family. Plays a role in cellular proliferation. Its function is limited to cell cycle G2-M phase. At least three alternatively spliced isoforms have been reported, two of which contain multiple non-AUG translation initiation sites.

Protein type: Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Kinase, protein; EC 2.7.11.22; CMGC group; CDK family; CDK/CDK10 subfamily; CDK10 subfamily

Chromosomal Location of Human Ortholog: 16q24

Molecular Function: protein binding; cyclin-dependent protein kinase activity; ATP binding

Biological Process: negative regulation of cell proliferation; traversing start control point of mitotic cell cycle; positive regulation of MAPKKK cascade; protein amino acid phosphorylation