PRM2 sirna

PRM2 siRNA (Human)

Cat. Num. AAA8230697

• Gene Names: PRM2; CT94.2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRM2; PRM2 siRNA (Human); Protamine-2; Sperm histone P2; Sperm protamine P2; PRM2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRM2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 140

• Applicable Applications for PRM2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRM2 sirna

siRNA to inhibit PRM2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 556503330
• NCBI GeneID: 5620
• NCBI Accession #: NP_001273285.1
• NCBI GenBank Nucleotide #: NM_001286356.1
• UniProt Accession #: P04554; Q6ZMM0
• Molecular Weight: 17,106 Da
• NCBI Official Full Name: protamine-2 isoform 2
• NCBI Official Synonym Full Names: protamine 2
• NCBI Official Symbol: PRM2
• NCBI Official Synonym Symbols: CT94.2
• NCBI Protein Information: protamine-2
• UniProt Protein Name: Protamine-2
• UniProt Gene Name: PRM2
• UniProt Synonym Gene Names: P2'
• UniProt Entry Name: PRM2_HUMAN

NCBI Description

Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis, and are the major DNA-binding proteins in the nucleus of sperm in many vertebrates. They package the sperm DNA into a highly condensed complex in a volume less than 5% of a somatic cell nucleus. Many mammalian species have only one protamine (protamine 1); however, a few species, including human and mouse, have two. This gene encodes protamine 2, which is cleaved to give rise to a family of protamine 2 peptides. Alternatively spliced transcript variants have also been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

PRM2: Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. Belongs to the protamine P2 family.

Protein type: DNA-binding; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 16p13.2

Cellular Component: nucleoplasm; nucleosome; nucleus

Molecular Function: DNA binding

Biological Process: chromosome condensation; multicellular organismal development; spermatogenesis; cell differentiation; DNA packaging