XRCC5 sirna

XRCC5 siRNA (Mouse)

Cat. Num. AAA8230654

• Gene Names: Xrcc5; Ku80; Ku86; AI314015
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: XRCC5; XRCC5 siRNA (Mouse); G22P2; X-ray repair cross-complementing protein 5; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku autoantigen protein p86 homolog; Ku80; Nuclear factor IV; XRCC5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: XRCC5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 732

• Applicable Applications for XRCC5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse XRCC5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for XRCC5 sirna

siRNA to inhibit XRCC5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 160333605
• NCBI GeneID: 22596
• NCBI Accession #: NP_033559.2
• NCBI GenBank Nucleotide #: NM_009533.2
• UniProt Accession #: P27641; Q3TE46; Q3TJT0; Q3TN82; Q80UT1; Q8C4N6; Q8K1K7; Q9R169
• Molecular Weight: 83,057 Da
• NCBI Official Full Name: X-ray repair cross-complementing protein 5
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 5
• NCBI Official Symbol: Xrcc5
• NCBI Official Synonym Symbols: Ku80; Ku86; AI314015
• NCBI Protein Information: X-ray repair cross-complementing protein 5
• UniProt Protein Name: X-ray repair cross-complementing protein 5
• UniProt Gene Name: Xrcc5
• UniProt Synonym Gene Names: G22p2; CTC85; CTCBF
• UniProt Entry Name: XRCC5_MOUSE

Uniprot Description

Ku80: the 80-kilodalton subunit of the Ku complex, also known as ATP-dependant DNA helicase II. A single stranded DNA-dependent ATP-dependent helicase. It functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.

Protein type: Nucleolus; RNA-binding; Helicase; DNA-binding; EC 3.6.4.-; EC 3.6.1.-; Nuclear receptor co-regulator

Cellular Component: nucleoplasm; protein complex; nuclear chromosome, telomeric region; membrane; cytoplasm; plasma membrane; chromosome; nucleus

Molecular Function: protein C-terminus binding; ATP-dependent DNA helicase activity; hydrolase activity, acting on acid anhydrides; DNA binding; hydrolase activity; ubiquitin protein ligase binding; double-stranded DNA binding; damaged DNA binding; nucleotide binding; telomeric DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; helicase activity; ATP binding

Biological Process: cell proliferation; positive regulation of neurogenesis; transcription, DNA-dependent; regulation of transcription, DNA-dependent; double-strand break repair; DNA repair; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; telomere maintenance; double-strand break repair via nonhomologous end joining; DNA recombination