ERBB4 sirna

ERBB4 siRNA (Rat)

Cat. Num. AAA8230562

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERBB4; ERBB4 siRNA (Rat); TYRO-2; Receptor tyrosine-protein kinase erbB-4; Proto-oncogene-like protein c-ErbB-4; ERBB4 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ERBB4 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1308

• Applicable Applications for ERBB4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ERBB4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERBB4 sirna

siRNA to inhibit ERBB4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11067415
• NCBI GeneID: 59323
• NCBI Accession #: NP_067719.1
• NCBI GenBank Nucleotide #: NM_021687.1
• UniProt Accession #: Q62956; Q6UA28; Q6UA29; Q9Z2N7
• Molecular Weight: 145,376 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-4
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 4
• NCBI Official Symbol: Erbb4
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-4
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-4
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: Erbb4
• UniProt Synonym Gene Names: Tyro-2; 4ICD; E4ICD
• UniProt Entry Name: ERBB4_RAT

NCBI Description

binds neuregulins; involved in epithelial-mesenchymal interactions during development; may play a role in tooth morphogenesis [RGD, Feb 2006]

Uniprot Description

HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported.

Protein type: Protein kinase, tyrosine (receptor); EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; TK group; EGFR family

Cellular Component: mitochondrion; basolateral plasma membrane; cytoplasm; postsynaptic density; integral to membrane; plasma membrane; caveola; nucleus; receptor complex; lipid raft

Molecular Function: protein binding; protein homodimerization activity; protein-tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; epidermal growth factor receptor binding

Biological Process: lactation; synapse maturation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; heart development; signal transduction; embryonic pattern specification; olfactory bulb interneuron differentiation; regulation of cell migration; odontogenesis; negative regulation of cell proliferation; positive regulation of glucose import; mammary gland development; positive regulation of cell proliferation; neural crest cell migration; positive regulation of cardiac muscle cell proliferation; response to drug; nervous system development; cell migration; cell fate commitment; transcription, DNA-dependent; positive regulation of synaptic transmission, GABAergic; positive regulation of phosphoinositide 3-kinase activity; telencephalon development; central nervous system morphogenesis; response to hydrogen peroxide; positive regulation of tyrosine phosphorylation of Stat5 protein; mitochondrial fragmentation during apoptosis; response to progesterone stimulus; surfactant homeostasis; positive regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration; negative regulation of apoptosis