CST8 sirna

CST8 siRNA (Human)

Cat. Num. AAA8230426

• Gene Names: CST8; CRES; CTES5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CST8; CST8 siRNA (Human); CRES; Cystatin-8; Cystatin-related epididymal spermatogenic protein; CST8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CST8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 142

• Applicable Applications for CST8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CST8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CST8 sirna

siRNA to inhibit CST8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 528524499
• NCBI GeneID: 10047
• NCBI Accession #: NP_001268659.1
• NCBI GenBank Nucleotide #: NM_001281730.1
• UniProt Accession #: O60676; Q2M2X6
• Molecular Weight: 16,275 Da
• NCBI Official Full Name: cystatin-8
• NCBI Official Synonym Full Names: cystatin 8 (cystatin-related epididymal specific)
• NCBI Official Symbol: CST8
• NCBI Official Synonym Symbols: CRES; CTES5
• NCBI Protein Information: cystatin-8
• UniProt Protein Name: Cystatin-8
• Protein Family: Cystatin
• UniProt Gene Name: CST8
• UniProt Synonym Gene Names: CRES
• UniProt Entry Name: CST8_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The encoded protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

CST8: Performs a specialized role during sperm development and maturation. Belongs to the cystatin family.

Protein type: Cell surface; Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: cell surface; cytoplasm; extracellular region

Molecular Function: cysteine protease inhibitor activity