EIF5A sirna

EIF5A siRNA (Mouse)

Cat. Num. AAA8229260

• Gene Names: Eif5a; Eif4d; Eif5a1; eIF-4D; eIF-5A; eIF-5A1; AA410058; eIF-5A-1; D19Wsu54e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EIF5A; EIF5A siRNA (Mouse); Eukaryotic translation initiation factor 5A-1; eIF-5A-1; eIF-5A1; Eukaryotic initiation factor 5A isoform 1; eIF-5A; eIF-4D; EIF5A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EIF5A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 154

• Applicable Applications for EIF5A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EIF5A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EIF5A sirna

siRNA to inhibit EIF5A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 262359885
• NCBI GeneID: 276770
• NCBI Accession #: NP_001160061.1
• NCBI GenBank Nucleotide #: NM_001166589.1
• UniProt Accession #: P63242; P10159; Q16182; Q5NCX0; Q78NR3; Q9D0G2
• Molecular Weight: 16,832 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 5A-1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 5A
• NCBI Official Symbol: Eif5a
• NCBI Official Synonym Symbols: Eif4d; Eif5a1; eIF-4D; eIF-5A; eIF-5A1; AA410058; eIF-5A-1; D19Wsu54e
• NCBI Protein Information: eukaryotic translation initiation factor 5A-1
• UniProt Protein Name: Eukaryotic translation initiation factor 5A-1
• Protein Family: Translation initiation factor
• UniProt Gene Name: Eif5a
• UniProt Synonym Gene Names: eIF-5A-1; eIF-5A1; eIF-5A
• UniProt Entry Name: IF5A1_MOUSE

NCBI Description

This gene encodes an elongation initiation factor, which participates in protein synthesis. The encoded protein also plays roles in mRNA metabolism, cell proliferation, and cell cycle control. This protein contains a modified lysine residue called hypusine, which appears to be necessary for its function. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 2, 5, and 19. [provided by RefSeq, Oct 2009]

Uniprot Description

EIF5A: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. Belongs to the eIF-5A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation initiation; Translation

Cellular Component: membrane; cell soma; endoplasmic reticulum; cytoplasm; dendrite; nuclear pore; nucleus; annulate lamellae

Molecular Function: RNA binding; ribosome binding; protein N-terminus binding; U6 snRNA binding; translation elongation factor activity

Biological Process: mRNA transport; translational frameshifting; translation; positive regulation of apoptosis; apoptosis; positive regulation of translational elongation; protein transport; mRNA export from nucleus; translational elongation; transport; positive regulation of cell proliferation; positive regulation of muscle cell differentiation; protein export from nucleus; positive regulation of translational termination; negative regulation of apoptosis