TGFB2 sirna

TGFB2 siRNA (Mouse)

Cat. Num. AAA8228634

• Gene Names: Tgfb2; Tgfb-2; BB105277; Tgf-beta2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TGFB2; TGFB2 siRNA (Mouse); Transforming growth factor beta-2; TGF-beta-2; TGFB2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TGFB2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 414

• Applicable Applications for TGFB2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TGFB2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TGFB2 sirna

siRNA to inhibit TGFB2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 166706905
• NCBI GeneID: 21808
• NCBI Accession #: NP_033393.2
• NCBI GenBank Nucleotide #: NM_009367.3
• UniProt Accession #: P27090; Q91VP5
• Molecular Weight: 47,589 Da
• NCBI Official Full Name: transforming growth factor beta-2
• NCBI Official Synonym Full Names: transforming growth factor, beta 2
• NCBI Official Symbol: Tgfb2
• NCBI Official Synonym Symbols: Tgfb-2; BB105277; Tgf-beta2
• NCBI Protein Information: transforming growth factor beta-2
• UniProt Protein Name: Transforming growth factor beta-2
• Protein Family: Transforming growth factor
• UniProt Gene Name: Tgfb2
• UniProt Synonym Gene Names: TGF-beta-2; LAP
• UniProt Entry Name: TGFB2_MOUSE

Uniprot Description

TGFB2: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Homodimer; disulfide-linked. Heterodimers with TGFB1 and with TGFB3 have been found in bone. Interacts with the serine proteases, HTRA1 and HTRA3. Interacts with ASPN. Belongs to the TGF-beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell development/differentiation; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; extracellular space; cell surface; cell soma; axon; cytoplasm; extracellular region; basement membrane; trans-Golgi network; endosome; secretory granule

Molecular Function: protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; beta-amyloid binding; punt binding; cytokine activity; protein N-terminus binding; transforming growth factor beta receptor binding; receptor signaling protein serine/threonine kinase activity; receptor binding

Biological Process: heart morphogenesis; extracellular matrix organization and biogenesis; collagen fibril organization; catagen; positive regulation of apoptosis; heart development; SMAD protein nuclear translocation; dopamine biosynthetic process; protein amino acid phosphorylation; regulation of apoptosis; hair follicle development; transforming growth factor beta receptor signaling pathway; somatic stem cell division; cell growth; cell cycle arrest; cartilage condensation; response to drug; negative regulation of keratinocyte differentiation; negative regulation of immune response; neuron fate commitment; positive regulation of cell cycle; positive regulation of catagen; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; cardioblast differentiation; positive regulation of protein secretion; response to estrogen stimulus; regulation of MAPKKK cascade; positive regulation of cell division; activation of protein kinase activity; neuron development; response to progesterone stimulus; positive regulation of heart contraction; negative regulation of apoptosis; cell death; axon guidance; wound healing; cell morphogenesis; cardiac muscle cell proliferation; positive regulation of stress-activated MAPK cascade; negative regulation of cell proliferation; negative regulation of release of sequestered calcium ion into cytosol; positive regulation of neuron apoptosis; positive regulation of cell proliferation; hemopoiesis; embryonic neurocranium morphogenesis; positive regulation of integrin biosynthetic process; skeletal development; negative regulation of epithelial cell proliferation; intercellular junction assembly and maintenance; blood vessel development; cell migration; regulation of transforming growth factor-beta2 production; hair follicle morphogenesis; positive regulation of cell adhesion mediated by integrin; glial cell migration; regulation of cell proliferation; eye development; positive regulation of cardioblast differentiation; response to hypoxia; epithelial to mesenchymal transition; blood vessel remodeling; negative regulation of cell growth; positive regulation of cell migration; growth