IQGAP2 sirna

IQGAP2 siRNA (Human)

Cat. Num. AAA8228064

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IQGAP2; IQGAP2 siRNA (Human); Ras GTPase-activating-like protein IQGAP2; IQGAP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IQGAP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1525

• Applicable Applications for IQGAP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IQGAP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IQGAP2 sirna

siRNA to inhibit IQGAP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 550544224
• NCBI GeneID: 10788
• NCBI Accession #: NP_001272389.1
• NCBI GenBank Nucleotide #: NM_001285460.1
• UniProt Accession #: Q13576; A8K4V1; B7Z8A4; J3KR91
• Molecular Weight: 122,806 Da
• NCBI Official Full Name: ras GTPase-activating-like protein IQGAP2 isoform 2
• NCBI Official Synonym Full Names: IQ motif containing GTPase activating protein 2
• NCBI Official Symbol: IQGAP2
• NCBI Protein Information: ras GTPase-activating-like protein IQGAP2
• UniProt Protein Name: Ras GTPase-activating-like protein IQGAP2
• Protein Family: Ras GTPase-activating-like protein
• UniProt Gene Name: IQGAP2
• UniProt Entry Name: IQGA2_HUMAN

NCBI Description

This gene encodes a member of the IQGAP family. The protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. [provided by RefSeq, Jul 2008]

Uniprot Description

IQGAP2: Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin. Isoform 2 expression is enhanced in testis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: filamentous actin; microtubule; microvillus; cell surface; lamellipodium; cytoplasm; actin cytoskeleton; filopodium

Molecular Function: calmodulin binding; actin filament binding; phosphatidylinositol-3,4,5-triphosphate binding; Rho GTPase binding; Rac GTPase binding; actin binding; GTPase inhibitor activity

Biological Process: small GTPase mediated signal transduction; negative regulation of catalytic activity; signal transduction