SF1 sirna

SF1 siRNA (Human)

Cat. Num. AAA8227976

• Gene Names: SF1; BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SF1; SF1 siRNA (Human); ZFM1; ZNF162; Splicing factor 1; Mammalian branch point-binding protein; BBP; mBBP; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162; SF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 613

• Applicable Applications for SF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SF1 sirna

siRNA to inhibit SF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 295842330
• NCBI GeneID: 7536
• NCBI Accession #: NP_001171501.1
• NCBI GenBank Nucleotide #: NM_001178030.1
• UniProt Accession #: Q15637; Q14818; Q14819; Q15913; Q8IY00; Q92744; Q92745; Q969H7; Q9BW01; Q9UEI0; B7Z1Q1; C9JJE2
• Molecular Weight: 65,371 Da
• NCBI Official Full Name: splicing factor 1 isoform 6
• NCBI Official Synonym Full Names: splicing factor 1
• NCBI Official Symbol: SF1
• NCBI Official Synonym Symbols: BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25
• NCBI Protein Information: splicing factor 1
• UniProt Protein Name: Splicing factor 1
• Protein Family: Splicing factor
• UniProt Gene Name: SF1
• UniProt Synonym Gene Names: ZFM1; ZNF162; BBP; mBBP
• UniProt Entry Name: SF01_HUMAN

NCBI Description

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

SF1: a splicing factor necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor. Phosphorylation of SF1 by cGMP-dependent protein kinase regulates spliceosome assembly. Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15. Six alternatively spliced isoforms have been described.

Protein type: Transcription, coactivator/corepressor; Spliceosome; RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; spliceosome; ribosome; nucleus

Molecular Function: zinc ion binding; RNA binding; transcription corepressor activity

Biological Process: nuclear mRNA 3'-splice site recognition; nuclear mRNA splicing, via spliceosome; Leydig cell differentiation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of smooth muscle cell proliferation; male sex determination; spliceosome assembly; regulation of steroid biosynthetic process