CDC7 sirna

CDC7 siRNA (Mouse)

Cat. Num. AAA8227321

• Gene Names: Cdc7; Cdc7l1; muCdc7; AI597260
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDC7; CDC7 siRNA (Mouse); CDC7L1; Cell division cycle 7-related protein kinase; CDC7-related kinase; muCdc7; CDC7 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CDC7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 532

• Applicable Applications for CDC7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CDC7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDC7 sirna

siRNA to inhibit CDC7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 409168308
• NCBI GeneID: 12545
• NCBI Accession #: NP_001258495.1
• NCBI GenBank Nucleotide #: NM_001271566.1
• UniProt Accession #: Q9Z0H0; Q9WUV1; Q9Z2Y7
• Molecular Weight: 59,203 Da
• NCBI Official Full Name: cell division cycle 7-related protein kinase isoform 1
• NCBI Official Synonym Full Names: cell division cycle 7 (S. cerevisiae)
• NCBI Official Symbol: Cdc7
• NCBI Official Synonym Symbols: Cdc7l1; muCdc7; AI597260
• NCBI Protein Information: cell division cycle 7-related protein kinase
• UniProt Protein Name: Cell division cycle 7-related protein kinase
• Protein Family: Cell division control protein
• UniProt Gene Name: Cdc7
• UniProt Synonym Gene Names: Cdc7l1; CDC7-related kinase; muCdc7
• UniProt Entry Name: CDC7_MOUSE

Uniprot Description

CDC7: an evolutionarily conserved serine/threonine protein kinase of the CDC7 family. Predominantly localized in the nucleus, it plays a pivotal role in linking cell cycle regulation to genome duplication, being essential for the firing of DNA replication origins. Plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. The human protein requires phosphorylation by CDK1 at T376 and binding to activator of S-phase kinase (ASK)/DBF4B to be fully activated. The interaction between Cdc7 and DBFK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. Although CDC7 expression levels appear to be constant throughout the cell cycle, the expression levels of DBF4B is cell cycle-regulated. Thus, the CDC7 activity is activated during S phase in eukaryotes. The Cdc7/ DBF4B complex regulates initiation of DNA replication by phosphorylating chromatin-bound mini-chromosome maintenance protein 2 (MCM2). Impaired Cdc7 kinase activity leads to S-phase arrest. Plays a role in the TGF-beta regulation of smooth muscle cell (SMC) differentiation. Interacts with Smad3 to facilitate Smad3 binding to SMC marker promoter, leading to the activation of SMC marker gene transcription.

Protein type: Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; CDC7 family

Cellular Component: microtubule cytoskeleton; nucleoplasm; intercellular bridge; cytoplasm; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: regulation of cell shape; peptidyl-serine phosphorylation; DNA replication initiation; cell division; positive regulation of cell proliferation; cell cycle; phosphorylation; protein amino acid phosphorylation; phagocytosis; double-strand break repair via break-induced replication