FCGR3B sirna

FCGR3B siRNA (Human)

Cat. Num. AAA8227202

• Gene Names: FCGR3B; CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FCGR3B; FCGR3B siRNA (Human); CD16B; FCG3; FCGR3; IGFR3; Low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma RIII-beta; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb; FcR-10; IgG Fc receptor III-1; CD antigen CD16b; FCGR3B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FCGR3B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 233

• Applicable Applications for FCGR3B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FCGR3B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FCGR3B sirna

siRNA to inhibit FCGR3B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 132814489
• NCBI GeneID: 2215
• NCBI Accession #: NP_000561.3
• NCBI GenBank Nucleotide #: NM_000570.4
• UniProt Accession #: O75015
• Molecular Weight: 26,216 Da
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor III-B isoform 2
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
• NCBI Official Symbol: FCGR3B
• NCBI Official Synonym Symbols: CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III-B
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III-B
• Protein Family: Low affinity immunoglobulin gamma Fc region receptor
• UniProt Gene Name: FCGR3B
• UniProt Synonym Gene Names: CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb
• UniProt Entry Name: FCG3B_HUMAN

NCBI Description

The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]

Uniprot Description

Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.