RUVBL2 sirna

RUVBL2 siRNA (Human)

Cat. Num. AAA8227104

• Gene Names: RUVBL2; RVB2; TIH2; ECP51; TIP48; CGI-46; INO80J; REPTIN; TIP49B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RUVBL2; RUVBL2 siRNA (Human); INO80J; TIP48; TIP49B; RuvB-like 2; 48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; ECP-51; INO80 complex subunit J; Repressing pontin 52; Reptin 52; TIP49b; TIP60-associated protein 54-beta; TAP54-beta; RUVBL2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RUVBL2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 418

• Applicable Applications for RUVBL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RUVBL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RUVBL2 sirna

siRNA to inhibit RUVBL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5730023
• NCBI GeneID: 10856
• NCBI Accession #: NP_006657.1
• NCBI GenBank Nucleotide #: NM_006666.1
• UniProt Accession #: Q9Y230; Q6FIB9; Q6PK27; Q9Y361; B3KQ59; E7ETE5
• Molecular Weight: 46,306 Da
• NCBI Official Full Name: ruvB-like 2
• NCBI Official Synonym Full Names: RuvB-like AAA ATPase 2
• NCBI Official Symbol: RUVBL2
• NCBI Official Synonym Symbols: RVB2; TIH2; ECP51; TIP48; CGI-46; INO80J; REPTIN; TIP49B
• NCBI Protein Information: ruvB-like 2
• UniProt Protein Name: RuvB-like 2
• Protein Family: RuvB
• UniProt Gene Name: RUVBL2
• UniProt Synonym Gene Names: INO80J; TIP48; TIP49B; 48 kDa TBP-interacting protein; ECP-51; Reptin 52; TAP54-beta
• UniProt Entry Name: RUVB2_HUMAN

NCBI Description

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq, Jul 2008]

Uniprot Description

RUVBL2: Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Forms homohexameric rings (Probable). Can form a dodecamer with RUVBL1 made of two stacked hexameric rings; however, even though RUVBL1 and RUVBL2 are present in equimolar ratio, the oligomeric status of each hexamer is not known. Oligomerization may regulate binding to nucleic acids and conversely, binding to nucleic acids may affect the dodecameric assembly. Interacts with the transcriptional activation domain of MYC. Interacts With ATF2. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL2 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with NPAT. Component of the chromatin- remodeling INO80 complex; specifically part of a complex module associated with the helicase ATP-binding and the helicase C- terminal domain of INO80. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with IGHMBP2. Interacts with TELO2. Ubiquitously expressed. Highly expressed in testis and thymus. Belongs to the RuvB family.

Protein type: Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; NuA4 histone acetyltransferase complex; nuclear matrix; membrane; cytoplasm; intracellular; nucleus; ribonucleoprotein complex

Molecular Function: ATP-dependent DNA helicase activity; identical protein binding; protein binding; DNA helicase activity; chromatin DNA binding; ATP-dependent 5'-3' DNA helicase activity; ATPase activity; unfolded protein binding; damaged DNA binding; ATP binding

Biological Process: chromatin remodeling; establishment and/or maintenance of chromatin architecture; protein folding; transcription, DNA-dependent; regulation of growth; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; DNA repair; DNA duplex unwinding; DNA recombination