CCDC88A sirna

CCDC88A siRNA (Human)

Cat. Num. AAA8226971

• Gene Names: CCDC88A; APE; GIV; GRDN; HkRP1; GIRDIN; KIAA1212
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCDC88A; CCDC88A siRNA (Human); APE; GRDN; KIAA1212; Girdin; Akt phosphorylation enhancer; Coiled-coil domain-containing protein 88A; G alpha-interacting vesicle-associated protein; GIV; Girders of actin filament; Hook-related protein 1; HkRP1; CCDC88A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CCDC88A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,796

• Applicable Applications for CCDC88A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCDC88A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCDC88A sirna

siRNA to inhibit CCDC88A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 208022632
• NCBI GeneID: 55704
• NCBI Accession #: NP_001129069.1
• NCBI GenBank Nucleotide #: NM_001135597.1
• UniProt Accession #: Q3V6T2; Q53SF1; Q581G3; Q5HYD0; Q7Z339; Q7Z3C5; A1IGE7; B7ZM78; C9JG83
• Molecular Weight: 207,797 Da
• NCBI Official Full Name: girdin isoform 1
• NCBI Official Synonym Full Names: coiled-coil domain containing 88A
• NCBI Official Symbol: CCDC88A
• NCBI Official Synonym Symbols: APE; GIV; GRDN; HkRP1; GIRDIN; KIAA1212
• NCBI Protein Information: girdin
• UniProt Protein Name: Girdin
• Protein Family: Girdin
• UniProt Gene Name: CCDC88A
• UniProt Synonym Gene Names: APE; GIV; HkRP1
• UniProt Entry Name: GRDN_HUMAN

NCBI Description

This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

girdin: a ubiquitously expressed actin binding protein that plays an important role in the formation of stress fibers and lamellipodia. Subsequent to phosphorylation by Akt, Girdin accumulates at the leading edge of migrating cells and appears to be crucial for the integrity of the actin cytoskeleton and for cell migration.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 2p16.1

Cellular Component: Golgi apparatus; membrane; endoplasmic reticulum; lamellipodium; cytoplasmic membrane-bound vesicle; plasma membrane; cytoplasmic vesicle; cytosol

Molecular Function: protein kinase B binding; protein homodimerization activity; microtubule binding; actin binding; phosphoinositide binding

Biological Process: lamellipodium biogenesis; regulation of protein amino acid phosphorylation; cell migration; TOR signaling pathway; regulation of actin cytoskeleton organization and biogenesis; activation of protein kinase B; DNA replication; regulation of cell proliferation; regulation of DNA replication