ALG2 sirna

ALG2 siRNA (Human)

Cat. Num. AAA8226840

• Gene Names: ALG2; CDGIi; CMS14; NET38; CMSTA3; hALPG2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALG2; ALG2 siRNA (Human); Alpha-1.3/1.6-mannosyltransferase ALG2; Asparagine-linked glycosylation protein 2 homolog; GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1.3-mannosyltransferase; GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase; GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1.6-mannosyltransferase; ALG2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ALG2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 323

• Applicable Applications for ALG2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ALG2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALG2 sirna

siRNA to inhibit ALG2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14861836
• NCBI GeneID: 85365
• NCBI Accession #: NP_149078.1
• NCBI GenBank Nucleotide #: NM_033087.3
• UniProt Accession #: Q9H553; Q8NBX2; Q8NC39; A2A2Y0
• Molecular Weight: 37,017 Da
• NCBI Official Full Name: alpha-1,3/1,6-mannosyltransferase ALG2
• NCBI Official Synonym Full Names: ALG2, alpha-1,3/1,6-mannosyltransferase
• NCBI Official Symbol: ALG2
• NCBI Official Synonym Symbols: CDGIi; CMS14; NET38; CMSTA3; hALPG2
• NCBI Protein Information: alpha-1,3/1,6-mannosyltransferase ALG2
• UniProt Protein Name: Alpha-1,3/1,6-mannosyltransferase ALG2
• Protein Family: Alpha-1,3/1,6-mannosyltransferase
• UniProt Gene Name: ALG2
• UniProt Entry Name: ALG2_HUMAN

NCBI Description

This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

ALG2: Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)- dolichol diphosphate. Defects in ALG2 are the cause of congenital disorder of glycosylation type 1I (CDG1I). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, integral; EC 2.4.1.132; EC 2.4.1.257; Glycan Metabolism - N-glycan biosynthesis

Chromosomal Location of Human Ortholog: 9q22.33

Cellular Component: endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; cytoplasm; integral to membrane; nucleus

Molecular Function: protein binding; glycolipid 3-alpha-mannosyltransferase activity; protein heterodimerization activity; glycolipid 6-alpha-mannosyltransferase activity; alpha-1,3-mannosyltransferase activity; protein N-terminus binding; calcium-dependent protein binding

Biological Process: protein amino acid glycosylation in endoplasmic reticulum; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; response to calcium ion

Disease: Congenital Disorder Of Glycosylation, Type Ii; Myasthenic Syndrome, Congenital, With Tubular Aggregates 3