Mouse HO-1 Monoclonal Antibody | anti-HO-1 antibody

HO-1 Antibody

Cat. Num. AAA8001884

• Gene Names: HMOX1; HO-1; HSP32; HMOX1D; bK286B10
• Reactivity: Human, Mouse, Rat, Bovine, Dog, Guinea Pig, Hamster, Monkey, Pig, Rabbit
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, ELISA
• Purity: Protein G Purified
• Synonyms: HO-1; Monoclonal Antibody; HO-1 Antibody; Mouse Anti-Human HO-1 Monoclonal IgG1 Kappa; Clone 1F12-A6; HSP32 Antibody; HMOX1 Antibody; Heme oxygenase 1 Antibody; HO Antibody; HO1 Antibody; 32 kD antibody; bK286B10 antibody; D8Wsu38e antibody; heat shock protein 32 kD antibody; heat shock protein 32kD antibody; Heat shock protein antibody; Heme oxygenase (decycling) 1 antibody; Heme oxygenase 1 antibody; Hemox antibody; HMOX 1 antibody; Hmox antibody; Hmox1 antibody; HMOX1_HUMAN antibody; HO 1 antibody; HO antibody; HO-1 antibody; HO1 antibody; Hsp32 antibody; anti-HO-1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine, Dog, Guinea Pig, Hamster, Monkey, Pig, Rabbit
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone Number: 1F12-A6
• Specificity: Detects 32kDa. Does not cross-react with HO-2.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 288

• Applicable Applications for anti-HO-1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: WB: 1:1000; IHC: 1:100; ICC/IF: 1:100
• Immunogen: Human HO-1 synthetic peptide, amino acids 1-30
• Immunogen Species: Human
• Conjugate: Unconjugated
• Cellular Localization: Microsome, Endoplasmic Reticulum
• Certificate of Analysis: 1 ug/ml was sufficient for detection of HO-1 in 10 ug of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6 . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Microsome. Endoplasmic reticulum. Localizes to the nucleus upon hypoxia. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6 . Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: muscle, dermis, hair follicles, epidermis: nuclear everywhere and some cytoplasmic staining.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6 . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Microsome. Endoplasmic reticulum. Localizes to the nucleus upon hypoxia. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite.)

Western Blot (WB)

(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6 . Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6 . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at-20 degree C. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cell-cell border staining in epidermis, punctuate nuclear staining.)

Related Product Information for anti-HO-1 antibody

Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).

Product Categories/Family for anti-HO-1 antibody

Cancer; Oxidative Stress; Neuroscience; Neurodegeneration; Alzheimer's Disease; Cardiovascular System; Blood; Platelets; Cell Signaling; Epigenetics and Nuclear Signaling; Nuclear Signaling Pathways; NFkB Pathway; Cardiovascular System; Atherosclerosis; V

References

1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411-419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925-10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759-1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129-135.

NCBI and Uniprot Product Information

• NCBI GI #: 4504437
• NCBI GeneID: 3162
• NCBI Accession #: NP_002124.1
• NCBI GenBank Nucleotide #: NM_002133.3
• UniProt Accession #: P09601
• NCBI Official Full Name: heme oxygenase 1
• NCBI Official Synonym Full Names: heme oxygenase 1
• NCBI Official Symbol: HMOX1
• NCBI Official Synonym Symbols: HO-1; HSP32; HMOX1D; bK286B10
• NCBI Protein Information: heme oxygenase 1
• UniProt Protein Name: Heme oxygenase 1
• UniProt Gene Name: HMOX1
• UniProt Synonym Gene Names: HO; HO1; HO-1
• UniProt Entry Name: HMOX1_HUMAN

NCBI Description

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]

Uniprot Description

HMOX1: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Expressed at higher levels in renal cancer tissue than in normal tissue. Belongs to the heme oxygenase family.

Protein type: EC 1.14.99.3; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Oxidoreductase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular space; endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; nucleolus; caveola; nucleus; cytosol

Molecular Function: protein binding; signal transducer activity; protein homodimerization activity; enzyme binding; metal ion binding; phospholipase D activity; heme binding; heme oxygenase (decyclizing) activity

Biological Process: cell death; response to nicotine; negative regulation of smooth muscle cell proliferation; cellular iron ion homeostasis; negative regulation of mast cell degranulation; positive regulation of smooth muscle cell proliferation; positive regulation of vasodilation; excretion; erythrocyte homeostasis; regulation of transcription factor activity; heme catabolic process; small GTPase mediated signal transduction; regulation of blood pressure; porphyrin metabolic process; negative regulation of mast cell cytokine production; angiogenesis; negative regulation of neuron apoptosis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; transmembrane transport; healing during inflammatory response; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription factor activity; heme oxidation; cellular response to nutrient; regulation of angiogenesis; negative regulation of leukocyte migration; negative regulation of DNA binding; iron ion homeostasis; positive regulation of angiogenesis; positive regulation of chemokine biosynthetic process; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; response to estrogen stimulus; endothelial cell proliferation; response to oxidative stress; smooth muscle hyperplasia

Disease: Heme Oxygenase 1 Deficiency; Pulmonary Disease, Chronic Obstructive