PIGZ sirna

PIGZ siRNA (Mouse)

Cat. Num. AAA8226836

• Gene Names: Pigz; F630022B06Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PIGZ; PIGZ siRNA (Mouse); GPI mannosyltransferase 4; GPI mannosyltransferase IV; GPI-MT-IV; Phosphatidylinositol-glycan biosynthesis class Z protein; PIG-Z; PIGZ sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PIGZ siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 560

• Applicable Applications for PIGZ sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PIGZ gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PIGZ sirna

siRNA to inhibit PIGZ expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 255003686
• NCBI GeneID: 239827
• NCBI Accession #: NP_766410.2
• NCBI GenBank Nucleotide #: NM_172822.3
• UniProt Accession #: Q8BTP0; Q149K0
• Molecular Weight: 62,043 Da
• NCBI Official Full Name: GPI mannosyltransferase 4
• NCBI Official Synonym Full Names: phosphatidylinositol glycan anchor biosynthesis, class Z
• NCBI Official Symbol: Pigz
• NCBI Official Synonym Symbols: F630022B06Rik
• NCBI Protein Information: GPI mannosyltransferase 4
• UniProt Protein Name: GPI mannosyltransferase 4
• UniProt Gene Name: Pigz
• UniProt Synonym Gene Names: GPI-MT-IV; PIG-Z
• UniProt Entry Name: PIGZ_MOUSE

Uniprot Description

PIGZ: Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers a fourth mannose to some trimannosyl-GPIs during GPI precursor assembly. The presence of a fourth mannose in GPI is facultative and only scarcely detected, suggesting that it only exists in some tissues. Belongs to the glycosyltransferase 22 family. PIGZ subfamily.

Protein type: Membrane protein, integral; EC 2.4.1.-; Transferase; Endoplasmic reticulum; Membrane protein, multi-pass; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis

Cellular Component: membrane; endoplasmic reticulum; integral to membrane

Molecular Function: oligosaccharyl transferase activity; alpha(1,6)-fucosyltransferase activity; acetylgalactosaminyltransferase activity; alpha(1,3)-fucosyltransferase activity; glucosyltransferase activity; O antigen polymerase activity; alpha-1,2-mannosyltransferase activity; acetylglucosaminyltransferase activity; transferase activity, transferring glycosyl groups; 1-phenanthrol glycosyltransferase activity; phenanthrol glycosyltransferase activity; glycolipid mannosyltransferase activity; galactosyltransferase activity; dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity; fucosyltransferase activity; dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity; lipopolysaccharide-1,6-galactosyltransferase activity; alpha-1,2-galactosyltransferase activity; alpha-1,3-galactosyltransferase activity; dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; 9-phenanthrol UDP-glucuronosyltransferase activity; UDP-glucose:glycoprotein glucosyltransferase activity; 1,2-dihydroxy-phenanthrene glycosyltransferase activity; dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; 9-phenanthrol glycosyltransferase activity; transferase activity; beta-1,4-mannosyltransferase activity; cellulose synthase activity; mannosyltransferase activity; alpha-1,3-mannosyltransferase activity; alpha-1,6-mannosyltransferase activity; inositol phosphoceramide synthase activity

Biological Process: GPI anchor biosynthetic process