Typical Testing Data/Standard Curve (for reference only)
Human CAM (Calmodulin) CLIA Kit | CAM clia kit
Human CAM (Calmodulin) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to CAM. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CAM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CAM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of CAM. You can calculate the concentration of CAM in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the EF-hand calcium-binding protein family. It is one of three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
Uniprot Description
Calmodulin: a calcium-binding small regulatory protein that mediates the control of a large number of enzymes by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Has four functional calcium-binding domains. Phosphorylation and ubiquitination result in a decreased activity.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 14q32.11
Cellular Component: nucleoplasm; spindle pole; centrosome; sarcomere; growth cone; spindle microtubule; cytoplasm; plasma membrane; extracellular region; nucleus; cytosol; vesicle
Molecular Function: protein domain specific binding; nitric-oxide synthase regulator activity; titin binding; calcium ion binding; protein kinase binding; calcium-dependent protein binding; protein serine/threonine kinase activator activity; protein binding; N-terminal myristoylation domain binding; phospholipase binding; thioesterase binding; type 3 metabotropic glutamate receptor binding; phosphoinositide 3-kinase binding; nitric-oxide synthase binding; adenylate cyclase binding
Biological Process: stimulatory C-type lectin receptor signaling pathway; positive regulation of phosphoprotein phosphatase activity; axon guidance; phototransduction, visible light; inositol phosphate metabolic process; activation of MAPKK activity; nerve growth factor receptor signaling pathway; signal transduction; nitric oxide metabolic process; positive regulation of protein amino acid dephosphorylation; synaptic transmission; substantia nigra development; platelet degranulation; muscle contraction; small GTPase mediated signal transduction; positive regulation of cyclic nucleotide metabolic process; response to corticosterone stimulus; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; glycogen catabolic process; regulation of heart rate; positive regulation of nitric-oxide synthase activity; response to amphetamine; adenylate cyclase activation; MAPKKK cascade; glucose metabolic process; regulation of cytokinesis; positive regulation of protein amino acid autophosphorylation; rhodopsin mediated signaling; G-protein coupled receptor protein signaling pathway; regulation of rhodopsin mediated signaling; phospholipase C activation; carbohydrate metabolic process; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; positive regulation of cyclic-nucleotide phosphodiesterase activity; detection of calcium ion; regulation of nitric-oxide synthase activity; response to calcium ion; blood coagulation; vascular endothelial growth factor receptor signaling pathway
Disease: Long Qt Syndrome 14; Ventricular Tachycardia, Catecholaminergic Polymorphic, 4
Research Articles on CAM
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Product Notes
The Human CAM calm1 (Catalog #AAA2531047) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531047 CLIA Kit recognizes Human CAM. It is sometimes possible for the material contained within the vial of "CAM (Calmodulin), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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