IMPG2 sirna

IMPG2 siRNA (Human)

Cat. Num. AAA8226670

• Gene Names: IMPG2; RP56; VMD5; IPM200; SPACRCAN
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IMPG2; IMPG2 siRNA (Human); IPM200; Interphotoreceptor matrix proteoglycan 2; Interphotoreceptor matrix proteoglycan of 200 kDa; IPM 200; Sialoprotein associated with cones and rods proteoglycan; Spacrcan; IMPG2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IMPG2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,241

• Applicable Applications for IMPG2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IMPG2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IMPG2 sirna

siRNA to inhibit IMPG2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 57242793
• NCBI GeneID: 50939
• NCBI Accession #: NP_057331.2
• NCBI GenBank Nucleotide #: NM_016247.3
• UniProt Accession #: Q9BZV3; Q9UKD4; Q9UKK5; A8MWT5
• Molecular Weight: 138,621 Da
• NCBI Official Full Name: interphotoreceptor matrix proteoglycan 2
• NCBI Official Synonym Full Names: interphotoreceptor matrix proteoglycan 2
• NCBI Official Symbol: IMPG2
• NCBI Official Synonym Symbols: RP56; VMD5; IPM200; SPACRCAN
• NCBI Protein Information: interphotoreceptor matrix proteoglycan 2
• UniProt Protein Name: Interphotoreceptor matrix proteoglycan 2
• Protein Family: Interphotoreceptor matrix proteoglycan
• UniProt Gene Name: IMPG2
• UniProt Synonym Gene Names: IPM200; IPM 200; Spacrcan
• UniProt Entry Name: IMPG2_HUMAN

NCBI Description

The protein encoded by this gene binds chondroitin sulfate and hyaluronan and is a proteoglycan. The encoded protein plays a role in the organization of the interphotoreceptor matrix and may promote the growth and maintenance of the light-sensitive photoreceptor outer segment. Defects in this gene are a cause of retinitis pigmentosa type 56 and maculopathy, IMPG2-related.[provided by RefSeq, Mar 2011]

Uniprot Description

IMPG2: Chondroitin sulfate- and hyaluronan-binding proteoglycan involved in the organization of interphotoreceptor matrix; may participate in the maturation and maintenance of the light- sensitive photoreceptor outer segment. Binds heparin. Defects in IMPG2 are the cause of retinitis pigmentosa type 56 (RP56). RP56 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in IMPG2 are the cause of maculopathy IMPG2- related (MACLP-IMPG2). MACLP-IMPG2 is a mild maculopathy characterized by full-field electroretinogram responses within normal limits, normal color vision, elevation of the photoreceptor layer in the foveal region and mild nuclear sclerosis.

Protein type: Extracellular matrix; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q12.2-q12.3

Cellular Component: proteinaceous extracellular matrix; integral to membrane; receptor complex

Molecular Function: heparin binding; extracellular matrix structural constituent; hyaluronic acid binding

Biological Process: visual perception

Disease: Retinitis Pigmentosa 56; Macular Dystrophy, Vitelliform, 5