CDC42BPA sirna

CDC42BPA siRNA (Rat)

Cat. Num. AAA8226662

• Gene Names: Cdc42bpa; Mrck; Pk428
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDC42BPA; CDC42BPA siRNA (Rat); PK428; Serine/threonine-protein kinase MRCK alpha; CDC42-binding protein kinase alpha; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; MRCK alpha; Myotonic dystrophy protein kinase-like alpha; CDC42BPA sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CDC42BPA siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: –

• Applicable Applications for CDC42BPA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CDC42BPA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDC42BPA sirna

siRNA to inhibit CDC42BPA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758474
• NCBI GeneID: 114116
• NCBI Accession #: NP_446109.1
• NCBI GenBank Nucleotide #: NM_053657.1
• UniProt Accession #: O54874
• Molecular Weight: – Da
• NCBI Official Full Name: serine/threonine-protein kinase MRCK alpha
• NCBI Official Synonym Full Names: CDC42 binding protein kinase alpha
• NCBI Official Symbol: Cdc42bpa
• NCBI Official Synonym Symbols: Mrck; Pk428
• NCBI Protein Information: serine/threonine-protein kinase MRCK alpha
• UniProt Protein Name: Serine/threonine-protein kinase MRCK alpha
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Cdc42bpa
• UniProt Synonym Gene Names: MRCK alpha; Myotonic dystrophy protein kinase-like alpha
• UniProt Entry Name: MRCKA_RAT

NCBI Description

catalyzes the phosphorylation of Ser19 of nonmuscle myosin light chain; binds GTP-Cdc42 and plays a role in cytoskeleton organization [RGD, Feb 2006]

Uniprot Description

MRCKA: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, AGC; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; DMPK family; GEK subfamily

Cellular Component: cell; leading edge; cytoplasm; actomyosin; intercellular junction

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding

Biological Process: regulation of small GTPase mediated signal transduction; cell migration; regulation of catalytic activity; actin cytoskeleton reorganization; actomyosin structure organization and biogenesis; cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis; nuclear migration; protein amino acid phosphorylation