Mouse anti-Human MAP4K5 Monoclonal Antibody | anti-MAP4K5 antibody

MAP4K5 (Mitogen-activated Protein Kinase Kinase Kinase Kinase 5, Kinase Homologous to SPS1/STE20, KHS, MAPK/ERK Kinase Kinase Kinase 5, MEK Kinase Kinase 5, MEKKK 5)

Cat. Num. AAA644277

• Gene Names: MAP4K5; KHS; GCKR; KHS1; MAPKKKK5
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MAP4K5; Monoclonal Antibody; MAP4K5 (Mitogen-activated Protein Kinase Kinase Kinase Kinase 5; Kinase Homologous to SPS1/STE20; KHS; MAPK/ERK Kinase Kinase Kinase 5; MEK Kinase Kinase 5; MEKKK 5); Anti -MAP4K5 (Mitogen-activated Protein Kinase Kinase Kinase Kinase 5; anti-MAP4K5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3H3
• Specificity: Recognizes human MAP4K5.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-MAP4K5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.
• Immunogen: Partial recombinant corresponding to aa361-470 from MAP4K5 (AAH36013) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (38.1kD).)

Western Blot (WB)

(Western Blot analysis of MAP4K5 expression in transfected 293T cell line by MAP4K5 monoclonal antibody.|Lane 1: MAP4K5 transfected lysate (95.04kD).|Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged MAP4K5 is ~0.03ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of MAP4K5 over-expressed 293 cell line, cotransfected with MAP4K5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP4K5 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Related Product Information for anti-MAP4K5 antibody

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.

Product Categories/Family for anti-MAP4K5 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 38570135
• NCBI GeneID: 11183
• NCBI Accession #: NP_942089.1
• NCBI GenBank Nucleotide #: NM_198794.2
• UniProt Accession #: Q9Y4K4; Q8IYF6
• Molecular Weight: 95,040 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 5
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 5
• NCBI Official Symbol: MAP4K5
• NCBI Official Synonym Symbols: KHS; GCKR; KHS1; MAPKKKK5
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 5; MEKKK 5; MEK kinase kinase 5; germinal center kinase-related; MAPK/ERK kinase kinase kinase 5; kinase homologous to SPS1/STE20
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 5
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K5
• UniProt Synonym Gene Names: KHS; MEK kinase kinase 5; MEKKK 5
• UniProt Entry Name: M4K5_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KHS1: a serine/threonine protein kinase of the STE20 family. Highly similar to yeast SPS1/STE20 kinase. Activates Jun kinase in mammalian cells, which suggested a role in stress response.

Protein type: Kinase, protein; Protein kinase, STE; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 14q11.2-q21

Cellular Component: cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: protein amino acid phosphorylation; activation of JNK activity