SLC35A1 sirna

SLC35A1 siRNA (Human)

Cat. Num. AAA8226261

• Gene Names: SLC35A1; CST; hCST; CDG2F; CMPST
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC35A1; SLC35A1 siRNA (Human); CMP-sialic acid transporter; CMP-SA-Tr; CMP-Sia-Tr; Solute carrier family 35 member A1; SLC35A1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC35A1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 278

• Applicable Applications for SLC35A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC35A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC35A1 sirna

siRNA to inhibit SLC35A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 270288773
• NCBI GeneID: 10559
• NCBI Accession #: NP_001161870.1
• NCBI GenBank Nucleotide #: NM_001168398.1
• UniProt Accession #: P78382; Q5W1L8
• Molecular Weight: 29,919 Da
• NCBI Official Full Name: CMP-sialic acid transporter isoform b
• NCBI Official Synonym Full Names: solute carrier family 35 (CMP-sialic acid transporter), member A1
• NCBI Official Symbol: SLC35A1
• NCBI Official Synonym Symbols: CST; hCST; CDG2F; CMPST
• NCBI Protein Information: CMP-sialic acid transporter
• UniProt Protein Name: CMP-sialic acid transporter
• Protein Family: CMP-sialic acid transporter
• UniProt Gene Name: SLC35A1
• UniProt Synonym Gene Names: CMP-SA-Tr; CMP-Sia-Tr
• UniProt Entry Name: S35A1_HUMAN

NCBI Description

The protein encoded by this gene is found in the membrane of the Golgi apparatus, where it transports nucleotide sugars into the Golgi. One such nucleotide sugar is CMP-sialic acid, which is imported into the Golgi by the encoded protein and subsequently glycosylated. Defects in this gene are a cause of congenital disorder of glycosylation type 2F (CDG2F). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]

Uniprot Description

SLC35A1: Transports CMP-sialic acid from the cytosol into Golgi vesicles where glycosyltransferases function. Defects in SLC35A1 are the cause of congenital disorder of glycosylation type 2F (CDG2F). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the nucleotide-sugar transporter family. SLC35A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: Golgi membrane; Golgi apparatus; integral to plasma membrane

Molecular Function: CMP-sialic acid transmembrane transporter activity; sugar:hydrogen ion symporter activity

Biological Process: proton transport; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; carbohydrate transport; carbohydrate metabolic process; protein modification process; protein amino acid N-linked glycosylation via asparagine; CMP-sialic acid transport; post-translational protein modification; transmembrane transport

Disease: Congenital Disorder Of Glycosylation, Type Iif