FOXC2 sirna

FOXC2 siRNA (Rat)

Cat. Num. AAA8226132

• Gene Names: Foxc2; Fkh14; Fkhl14; Hfhbf3
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FOXC2; FOXC2 siRNA (Rat); HFHBF3; Forkhead box protein C2; Brain factor 3; BF-3; HFH-BF-3; FOXC2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: FOXC2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 494

• Applicable Applications for FOXC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat FOXC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FOXC2 sirna

siRNA to inhibit FOXC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 156119589
• NCBI GeneID: 171356
• NCBI Accession #: NP_001095150.1
• NCBI GenBank Nucleotide #: NM_001101680.1
• UniProt Accession #: Q63246; M0R736
• Molecular Weight: 52,854 Da
• NCBI Official Full Name: forkhead box protein C2
• NCBI Official Synonym Full Names: forkhead box C2
• NCBI Official Symbol: Foxc2
• NCBI Official Synonym Symbols: Fkh14; Fkhl14; Hfhbf3
• NCBI Protein Information: forkhead box protein C2
• UniProt Protein Name: Forkhead box protein C2
• Protein Family: Forkhead box protein
• UniProt Gene Name: Foxc2
• UniProt Synonym Gene Names: Hfhbf3; BF-3
• UniProt Entry Name: FOXC2_RAT

NCBI Description

transcription factor, characterized by a distinct forkhead DNA-binding domain, function not yet known, may play a role in the development of mesenchymal tissues [RGD, Feb 2006]

Uniprot Description

FOXC2: Transcriptional activator. Might be involved in the formation of special mesenchymal tissues (By similarity)

Protein type: DNA-binding; Cell development/differentiation; Apoptosis; Transcription factor

Cellular Component: nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; DNA binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: heart morphogenesis; transcription from RNA polymerase II promoter; collagen fibril organization; neural crest cell development; paraxial mesoderm formation; heart development; positive regulation of transcription, DNA-dependent; response to hormone stimulus; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; ureteric bud development; lymphangiogenesis; ventricular cardiac muscle morphogenesis; kidney development; regulation of blood vessel size; skeletal development; blood vessel development; Notch signaling pathway; somitogenesis; ossification; camera-type eye development; positive regulation of cell adhesion mediated by integrin; regulation of organ growth; embryonic viscerocranium morphogenesis; embryonic cranial skeleton morphogenesis; embryonic skeletal morphogenesis; patterning of blood vessels; regulation of transcription from RNA polymerase II promoter; paraxial mesodermal cell fate commitment; cell proliferation; insulin receptor signaling pathway; artery morphogenesis; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; blood vessel remodeling; lymph vessel development; vascular endothelial growth factor receptor signaling pathway; metanephros development