TRIM32 sirna

TRIM32 siRNA (Human)

Cat. Num. AAA8225444

• Gene Names: TRIM32; HT2A; BBS11; TATIP; LGMD2H
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRIM32; TRIM32 siRNA (Human); HT2A; E3 ubiquitin-protein ligase TRIM32; 72 kDa Tat-interacting protein; Tripartite motif-containing protein 32; Zinc finger protein HT2A; TRIM32 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TRIM32 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 653

• Applicable Applications for TRIM32 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRIM32 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRIM32 sirna

siRNA to inhibit TRIM32 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 153791514
• NCBI GeneID: 22954
• NCBI Accession #: NP_001093149.1
• NCBI GenBank Nucleotide #: NM_001099679.1
• UniProt Accession #: Q13049; Q9NQP8
• Molecular Weight: 71,989 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM32
• NCBI Official Synonym Full Names: tripartite motif containing 32
• NCBI Official Symbol: TRIM32
• NCBI Official Synonym Symbols: HT2A; BBS11; TATIP; LGMD2H
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM32
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM32
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM32
• UniProt Synonym Gene Names: HT2A
• UniProt Entry Name: TRI32_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM32: Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo. Defects in TRIM32 are the cause of limb-girdle muscular dystrophy type 2H (LGMD2H); also known as muscular dystrophy Hutterite type. LGMD2H is an autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. Defects in TRIM32 are the cause of Bardet-Biedl syndrome type 11 (BBS11). Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect. Belongs to the TRIM/RBCC family.

Protein type: Ligase; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 9q33.1

Cellular Component: striated muscle thick filament; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; ubiquitin binding; myosin binding; protein self-association; zinc ion binding; RNA binding; Tat protein binding; translation initiation factor binding; transcription coactivator activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: fat cell differentiation; regulation of interferon type I production; positive regulation of neurogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein polyubiquitination; positive regulation of proteolysis; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of cell cycle; protein ubiquitination; positive regulation of cell growth; activation of NF-kappaB transcription factor; negative regulation of viral transcription; negative regulation of fibroblast proliferation; positive regulation of protein catabolic process; positive regulation of interferon type I production; innate immune response; positive regulation of transcription factor activity; positive regulation of neuron differentiation; response to UV; positive regulation of cell migration

Disease: Muscular Dystrophy, Limb-girdle, Type 2h; Bardet-biedl Syndrome 11