KDELC1 sirna

KDELC1 siRNA (Human)

Cat. Num. AAA8225436

• Gene Names: KDELC1; EP58; KDEL1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KDELC1; KDELC1 siRNA (Human); EP58; KDEL motif-containing protein 1; Endoplasmic reticulum resident protein 58; ER protein 58; ERp58; KDELC1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KDELC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 502

• Applicable Applications for KDELC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KDELC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KDELC1 sirna

siRNA to inhibit KDELC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 176866369
• NCBI GeneID: 79070
• NCBI Accession #: NP_076994.2
• NCBI GenBank Nucleotide #: NM_024089.2
• UniProt Accession #: Q6UW63; Q53HL3; Q9BVD2
• Molecular Weight: 58,043 Da
• NCBI Official Full Name: KDEL motif-containing protein 1
• NCBI Official Synonym Full Names: KDEL (Lys-Asp-Glu-Leu) containing 1
• NCBI Official Symbol: KDELC1
• NCBI Official Synonym Symbols: EP58; KDEL1
• NCBI Protein Information: KDEL motif-containing protein 1
• UniProt Protein Name: KDEL motif-containing protein 1
• Protein Family: KDEL motif-containing protein
• UniProt Gene Name: KDELC1
• UniProt Synonym Gene Names: EP58; ER protein 58; ERp58
• UniProt Entry Name: KDEL1_HUMAN

NCBI Description

This gene encodes a protein product localized to the lumen of the endoplasmic reticulum. As a member of the endoplasmic reticulum protein family the encoded protein contains a Lys-Asp-Glu-Leu or KDEL motif located at the extreme C-terminus which prevents all endoplasmic reticulum resident proteins from being secreted. Proteins carrying this motif are bound by a receptor in the Golgi apparatus so that the receptor-ligand complex returns to the endoplasmic reticulum. A processed non-transcribed pseudogene located in an intron of a sodium transporter gene on chromosome 5 has been defined for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KDELC1: a protein product localized to the lumen of the endoplasmic reticulum. As a member of the endoplasmic reticulum protein family the encoded protein contains a Lys-Asp-Glu-Leu or KDEL motif located at the extreme C-terminus which prevents all endoplasmic reticulum resident proteins from being secreted. Proteins carrying this motif are bound by a receptor in the Golgi apparatus so that the receptor-ligand complex returns to the endoplasmic reticulum. A processed non-transcribed pseudogene located in an intron of a sodium transporter gene on chromosome 5 has been defined for this gene. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q33

Cellular Component: endoplasmic reticulum lumen